XB-CLONE-4351513
Clone Name: IMAGE:7027039
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Gene:
sap18
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC080173
[+]
TGAGTGCAGAGCGCTGGACTTCGTGGTGTTTGTAGTTCTATTAATCGGTAAGGCTGGCTCTTTCCACCAAACTGGAGAAGATGGCGGTGGAATCGCGGGT
GACGCAGGAGGAGATAAAAAAGGAACCGGAGAAACCAATCGATAGAGAGAAGACCTGCCCTCTTCTCTTGAGGGTTTTCACAACAAACAACGGGAGACAC
CATCGCATGGATGAATTTTCGAGGGGAAACGTGCCATCCAGTGAGCTACAGATCTATACCTGGATGGATGCCACGCTGAAGGAATTAACAAGCTTAGTTA
AAGAAGTTTACCCAGATGCTCGGAAAAAAGGAACTCATTTCAACTTTGCTATTGTCTATCCGGATCCAAAGCGATCAGGCTACAGGGTGAAGGAGATTGG
GAGTACAATTTCAGGCAGAAAGGGTTCAGATGACTCCATGACACTTCAATCACAAAGATTTCAGATTGGAGACTATCTGGACATAGCAATTACACCTCCA
AACAGAGCTCCACCTCCTTCAGGGCGCATGAGGCCCTATTAACACATTTTTTTTTTTTTTTTTAAAGTGGACATTTTGCTTTTGTGATATCGGTTTTAAG
GTCCTGAAAAAAGCAATGATCTTCAGTTTCAATTTATTTGGTTTTGTGCATTTTAAAATCTTATAAAGTCTTTGTTTTTTTTTTTAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAATAAAAAAAAAAAAAAAA
5' EST: CF784844
[+]taattaatcccccccccccctgagtgcagagcgctggacttcgtggtgtttgtagttctattaatcggtaaggctggctctttccaccaaactggagaag
atggcggtggaatcgcgggtgacgcaggaggagataaaaaaggaaccggagaaaccaatcgatagagagaagacctgccctcttctcttgagggttttca
caacaaacaacgggagacaccatcgcatggatgaattttcgaggggaaacgtgccatccagtgagctacagatctatacctggatggatgccacgctgaa
ggaattaacaagcttagttaaagaagtttacccagatgctcggaaaaaaggaactcatttcaactttgctattgtctatccggatccaaagcgatcaggc
tacagggtgaaggagattgggagtacaatttcaggcagaaagggttcagatgactccatgacacttcaatcacaaagatttcagattggagactatctgg
acatagcaattacacctccaaacagagctccacctccttcagggcgcatgangccctattaacacatttttttttttttttttttaaagtggacaatttt
gccttttggggaaatccggtttttaaaggtccctggaaaaaaagccaatggattcttttccgttttcccaatttttatttttgggtttttttggggccct
tttttttaaaaaaatccttt
3' EST: CF784843
[+]tttttttttttttttattttttttttttttttttttttttttttttttttaaaaaaaaaaaacaaagactttataaaattttaaaatgcccaaaaccaaa
taaattgaaactgaaaatcattgcttttttcaggaccttaaaaccgatatcccaaaagcaaaatgtcccctttaaaaaaaaaaaaaaaaatgtgttaaaa
gggccccatgccccctgaaggagggggacccctgtttggaggtgtaattgctatgtccaaaaagtccccaatctgaaatctttgtgattgaagtgtcatg
gaatcatctaaaccctttctgcctgaaattgtacccccaatccccttcaccctgtaccctgatccctttggatccggaaaaacaataccaaagttgaaat
gagttccttttttccaaacatctgggtaaacttctttaactaaccttgttaattccttcagcggggcatccatccaggtaaaaatctgtaccccactgga
gggcacgtttccccccaaaaattcatccatgcaatggggtcccccgttgtttgttgggaaaacccccaaaaaaaaagggcaggtcttccccctatcgatt
ggtttccccggttccttttttatcccccccggggtcacccgcgattccaccgccatcttctccagtttggtggaaagacccccccttaccgattaataga
actacaaacaccccgaagtccagcgcctcaccagggggggggggggggggggggataaatttaaccccggggggcaaaaaaagaaccaattacccttgta
attccgggccccggaaaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: