XB-CLONE-4351507
Clone Name: IMAGE:7027036
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Gene:
macroh2a1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077015
[+]
CCCCGATAGGGGACAGTAGACATATTGCTGGGAACTGCATTAAATTCCAGCTTCTGATTACACTTAATCAAGATGTCAAGTAGAGGGGGTAAAAAGAAGA
CAACAAAGACCACGCGGTCTGCAAAGGCCGGGGTTATATTTCCAGTTGGGAGAATGCTGCGTTACATAAAGAAAGGGCACCCAAAATACAGAATTGGAGT
TGGTGCACCAGTATATATGGCAGCTGTACTGGAATATCTAACTGCGGAAATTTTGGAATTGGCTGGAAATGCAGCGAGAGACAACAAAAAGAGTCGAGTA
ACACCACGACATATACTTCTTGCAGTAGCGAATGATGAAGAATTAAATCAGCTATTGAAAGGGGTCACAATAGCAAGTGGTGGTGTTTTACCTAATATTC
ATCCAGAACTCCTGGCAAAGAAACGTGGATCCAAAGGAAAGCTGGAAGCCATCATTACTCCACCACCAGCTAAAAAGTCAAAGACAACATCAACTAAAAA
GACATTGGCAAAGAAACCTGGAGCCAAGAAAGGAGTTAGAAAATCTAAGAAAAAACAGGGAGACGTCAGCAAATCAGCAAGTGCAGATAGCACAACAGAT
GGCACACCTTCTGATGGATTCACTGTTCTTTCCACCAAAAGTCTTTTCCTCGGACAGAAGTTGCAAGTTGTAAAGGCTGACATTGCCACCATTGACAGTG
ATGCTGTCGTTCACCCGACAAGCTCTGATCTCTACACCGGTGGTGAAGTAGGTAATGCCTTAGAGAAGAAAGGTGGCAAAGAGTTTGTGGAAGCTGTTAC
TGAAAAAAAAAAAAAAAA
5' EST: CF784848
[+]taattaatcccccccccccccccccgataggggacagtagacatattgctgggaactgcattaaattccagcttctgattacacttaatcaagatgtcaa
gtagagggggtaaaaagaagacaacaaagaccacgcggtctgcaaaggccggggttatatttccagttgggagaatgctgcgttacataaagaaagggca
cccaaaatacagaattggagttggtgcaccagtatatatggcagctgtactggaatatctaactgcggaaattgtggaattggctggaaatgcagcgaga
gacaacaaaaagagtcgagtaacaccacgacatatacttcttgcagtagcgaatgatgaagaattaaatcagctattgaaaggggtcacaatagcaagtg
gtggtgttttacctaatattcatccagaactcctggcaaagaaacgtggatccaaaggaaagctggaagccatcattactccaccaccagctaaaaagtc
aaagacaacatcaactaaaaagacattggcaaaaaaaacctgggaaccaagaaaggagttagaaaatctaaaaaaaaaacaggggatacgttctgccaaa
tcagcaaagtggagaataggctcaacaggaagggcaccccctttctgatggaattcactggtttctttttccaccaaaatattttttttcccccgggaac
aaaaattttgcaagtttggaaaaaggccggaaaattttcccccccattgtgaacagtgtgaagcctggtctttttcctccccggaaaaaaggccctaaga
ttctcctcacccaccgggtgggttgaaaaagataagggtaatgagcccactcgaaaaat
3' EST: CF784847
[+]tttttttttttttttcagtaacagcttccacaaactctttgccacctttcttctctaaggcattacctacttcaccaccggtgtagagatcagagcttgt
cgggtgaacgacagcatcactgtcaatggtggcaatgtcagcctttacaacttgcaacttctgtccgaggaaaagacttttggtggaaagaacagtgaat
ccatcagaaggtgtgccatctgttgtgctatctgcacttgctgatttgctgacgtctccctgttttttcttagattttctaactcctttcttggctccag
gtttctttgccaatgtctttttagttgatgttgtctttgactttttagctggtggtggagtaatgatggcttccagctttcctttggatccacgtttctt
tgccaggagttctggatgaatattaggtaaaacaccaccacttgctattgtgacccctttcaatagctgatttaattcttcatcattcgctactgcaaga
agtatatggtcgtgggggttactcgaactccttttggttgccactcggctgcattttccagcccattttcaaaaattttccgccagttagaaatattccc
ggtaacaggctgcccatattatacgggggggcacccaaactcccaatttcctggaatgttggagggggcccctttttctttttatgtaaaccggcaagca
gttcttccccacatggggaaaaaataaaaaccctccggcgacctattgcacaaagacgtgcgggggaccccgttaggattggcccatcaatttatgaaac
caccctcaccttaatgtaaaaactactgggggagtataaccgtcaaatgtacgacagctcggaggn
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: