XB-CLONE-4351501
Clone Name: IMAGE:7027033
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Gene:
hmgn1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784852
[+]
taattaatccccccccccccggactagtctttgtgagtactccatctgcaggagggctcgcacatagaaaaaacaaatatatatatataaataagtgcaa
gcaagccagggatttaagtgtgtaggcgtccatttgcagagaaggctgctaagcgttcccgttgcttctgtctttggattcaagcggtgcacacaagccc
tcagtgcagccatgcctaagagaaagcaggtgaatgctgctgatgctgcagatgccaaggatgagcccaagaggagatcggcacggttgtcggctaaacc
ggccccaccaaaagctgaaccaaagccaaaaaaagacaaggcacccgtaaaggaaaagcctgaggagaacgacaaaaaagttccagcatagggtaaaaaa
gggagcctatgggcaaaccgaccgagggaatctaaccaagtaaaaaaccctaaggaagaactcgcctgtcctgaaaaacgggaacaaacaaaaaaagtgg
actaaaggcccccctggcttccttgaatgggaagggcctaaccaagggaaactccaaaagtttctcgaaaaataaaccctttccgttcctttgggaaacc
gaagatccttattttatatcattggggtttccctggttaacccccccccctttggtagcaaattttccaaaagagaaaaaatttttctttacccaaatag
atatattttgtgaaaaaattgccaaaatctttttttataagtattaccccctacatatttcccccgagacaaaaccacacactttgttcctcataaacaa
taatgcgagaggcgtatattcccccccancgccaaatatctttgtctattgggtaggtagagatatatacaaaacacatttctttatttttactaacacc
ctcct
3' EST: CF784851
[+]tttttttttttttttaaagttcagagagaagttgtttcgtagaaaatttagcacccctcgtaccagcatctttgatgcaaataattcaacccatttctcg
gccaagaaggagcgaacacgcataggtgccaccttggcaggaaagagatgcaaacacatgaaccgccgtgtcctttttgtttttctccctacttcacatc
acactcactacacactcaaagaagtttccccagaagagattcacagcacttttacaaagttactgaaaataaaataccactacccaggaaaaaaaaaaat
aaaaatggtaacatcacagatagtacacaatttctttagagggaaaatccggtcacctttggggagatttattaatttttaaaagtggaaaatttgtacc
ccagggaacaacctggagtacaaaagaaaacgtttaaaaaccttaaaaaacatttttaaataaaccccgtggaatttttggccccccaatttgttacccc
aattatcggggggcactgggccctggtttcaaaaccggggaaacccctgggcttttaagggttcctttgccccgtttttttggttcccccaaattttaaa
aaaaaaaaccctttttttaaaaacggggcccaaagggggttttgggaaatttcccccccaaagggaaaaaccgggggtttttggggggggggggaaaaaa
aaaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: