XB-CLONE-4351498
Clone Name: IMAGE:7027032
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Gene:
tecr
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC080144 [+]
GGTTTCCCCGCCCCCGGTTGAAGCTTAGTGGCAGGTAGAAGAGAAGCGTGTTTGCTCCAGGGGAGCCCTGCGGAATCATGAAACACCATGAGGTGGAGAT
CCTGGATGCCAAAACGCGGGAGAAGCTGTGCTTCTTAGACAAGGTGGAGCCCCATGCCACCATAGCAGATATAAAGAATATGTTCACCAAATCCCATCCT
CAGTGGTACCCTGCGCGGCAGTCTCTACGCCTGGATCCAAAGGGCAGATCGCTGAAGGATGAAGACATCTTACAGAACCTACCAGTGGGGACAACAGCCA
CCCTGTACTTTAGAGATCTGGGTGCACAGATCAGCTGGGTGACGGTGTTCCTCACAGAATACGCTGGGCCTCTCGTTATATATCTGCTCTTCTATTTCCG
AGTTCCTTTTATTTATGGTCCCAAGTATGATTTTATTACCAGCCGGCATTCTGTGGTACACCTGGCCTGTATCTGCCATTCTTTTCATTACATTAAACGT
CTACTGGAGACCCTGTTCGTCCATCGTTTCTCTCATGGTACAATGCCGTTCCGGAATATCTTTAAGAACTGTATGTATTACTGGGGATTTGCAGCTTGGA
TGGCTTACTACATAAACCACCCACTCTACACACCACCGACATATGGAGAAAATCAGGTGAAGCTGGCGGTCATTATATTTCTGTTTTGCCAGCTGGGTAA
TTTCTCCATACACATTGCTTTGCGGAATCTGCGACCTGCTGGTTCCAAGACCCGAAAGATTCCCTTCCCCACACGTAATCCTTTCACATGGCTGTTCCTG
CTGGTTTCCTGTCCCAACTACACATATGAGGTTGGCTCTTGGATTGGATTTGCCATCATGACCCAGTGTCTCCCAGTCGCTCTCTTTACCTTGGTTGGAT
TCATCCAGATGACGATTTGGGCCAAAGGCAAACACCGAAGTTACTTGAAGGAGTTCCGAGATTACCCTCCTCTCCGATCCCCCATAGTTCCCTTCATCCT
GTAGGCACACTCATGGTTCCCTCTGTTCCTTGCTCTTCCTTTCTAACTCTAGTTCTTAAAATGACCACCAATATCCAACTGTCTGCTAAACACTGGCCTC
TAAACTCTTGCACTGATATATAGAGTATGGAAGGTTTGTGCTTCCCAGATGGAATAAACGTGTCCTCTGTTAAAGAAAAAAAAAAAAAAAA
5' EST: CF784854 [+]taattaatcccccccccccccggtttccccgcccccggttgaagcttagtggcaggtagaagagaagcgtgtttgctccaggggagccctgcggaatcat
gaaacaccatgaggtggagatcctggatgccaaaacgcgggagaagctgtgcttcttagacaaggtggagccccatgccaccatagcagatataaagaat
atgttcaccaaatcccatcctcagtggtaccctgcgcggcagtctctacgcctggatccaaagggcagatcgctgaaggatgaagacatcttacagaacc
taccagtggggacaacagccaccctgtactttagagatctgggtgcacagatcagctgggtgacggtgttcctcacagaatacgctgggcctctcgttat
atatctgctcttctatttccgagttccttttatttatggtcccaagtatgattttattaccagccggcattctgtggtacacctggcctgtatctgccat
tcttttcattacattaaacgtctactggagaccctgttcgtccatcgtttctctcatggtacaatgccgttccggaatatctttaagaactgtatgtatt
actggggatttgcagcttggatggcttactacataaaccacccactctacacaccaccgacatatgggagaaaatcaggggaagctggcggccaattata
tttctggttttggccagctggggtaatttctcccttacacattggcttttgcggaaatctgccaa
3' EST: CF784853 [+]tttttttttttttttctttaacagaggacacgtttattccatctgggaagcacaaaccttccatactctatatatcagtgcaagagtttagaggccagtg
tttagcagacagttggatattggtggtcattttaagaactagagttagaaaggaagagcaaggaacagagggaaccatgagtgtgcctacaggatgaagg
gaactatgggggatcggagaggagggtaatctcggaactccttcaagtaacttcggtgtttgcctttggcccaaatcgtcatctggatgaatccaaccaa
ggtaaagagagcgactgggagacactgggtcatgatggcaaatccaatccaagagccaacctcatatgtgtagttgggacaggaaaccagcaggaacagc
catgtgaaaggattacgtgtggggaagggaatctttcgggtcttggaaccagcaggtcgcagattccgcaaagcaatgtgtatggagaaattacccagct
ggcaaaacagaaatataatgaccgccagcttcacctgattttctccatatgtcggtggtgtgtagagtgggtggtttatgtagtaagccatccaagctgc
aaatccccagtaatacatacagttcttaaagatattccggaacggcattgtaccatgagagaaacgatggacgaacagggtctccagtagacgtttaatg
taatgaaaagaatggcagatacaggccaggtgtaccacagaatgccggctgggaataaaatcatacttgggaccataaataaaaggaactccggaaatag
aagagccgatatttaac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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