XB-CLONE-4351490
Clone Name: IMAGE:7027029
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Gene:
prmt9
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784859
[+]
taattaatcccccccccccccgagagagtggtgcctgcagtgaagcattcacacagtcagcctcagagtgtaaaatgttttctgtaaactacacattact
aatggtaccctctgtttttttttacagatttaaatgaattgctctagtgagacttggtcagcatgtgcaattctcattataaagaatgaacgaggcggga
aaggaagtgcagccgagaggtagaatggcagcccaggctgctagcaagagacgcctcatctccaggtctttgcaaagtgctcagatctgcctgcagaatc
aggactatggcaccgcatatgcacaccttctgctagtgcttactctggctccagagcaaaaggatgccttaaaggatatgtttcagtattctttatttaa
gtgggcagaagagctgtatgctttgaatcgaagtcaagatttgttcaattgttatgagcaagcactagagcttttcccaagagatgatgtaatttgtaac
agtatgggcgaacatctcttcaggctgggcttccgagatgaagcagctggttatttctacaaggcactgaagctgaacccaagctctgctgagggcaaag
aaaacttctaccgtgtagcaaactggttgattaaacgctggcattttatcatgctcaatgaccacaagaagaaacctgatgtatcaagaaaactatccca
aatgccatttcaaaaagggtttgcaaaaatggtactctgaaaatttggaaaattgggcaccgggaaatttttaagccagggtttgccttaaaagaaagct
gggaagcctttccaaaaacaattatggccttgtgtgaaacctgttct
3' EST: CF784858
[+]tttttttttttttttggcttttcaaaagtaacaaaatatatacagtttatttgcttaaaaccacaagacttcctttaagatgacaattgctttgtaagaa
ttgagtcatctcactgcaatggtcttgttagtgccatgctgatactgctgttctggaactgaacctcaagcaagagctcctccccttccagcacactcat
aggggtctccagcacatacgctgcctgtttccaatgtgatgcctcactggatgtgtctagggtaatggcttcatcaaggtgtatgtggtaccagaatggg
acagcggttacctggccagatctacagatattcaccttgattctcattacagagctgctcagattgtttgtatagggattcattagatccagccttagca
gttcaaatgtttcacttaaggggatacagggtacagtagagaggttcaaaaatacatggacaggaaccttgaatcgattgatagcaggtgctataagaaa
gcctagagtaggctcggtgccttggacagagccctcctgcagtaatgtctttgattctaccagcatcccttgcattaccactgcatggggaaatatgttg
cctcctggctgtagcagacacctggctattgcagctttctctaataagtcttgtcgaatcagaacacaggtctcaatcacatcaagaaatatgatgctcc
acatcttgtcagactttggcttctgtaaagaatcatcatctgttttccgctgggcttaaacaaaatttccggaaactcattaattgaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: