XB-CLONE-4351482
Clone Name: IMAGE:7027025
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Gene:
h2az1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784864
[+]
taattaatcccccccccccccagactgtttcgagtgccagagcgccagtttgggtttaaggaggaattacaggacttgattaagataaaaatggctggtg
gcaaggctggcaaagataccggaaaagcaaaggctacctctatcacccgatcctccagagctggattgcagtttccagttggtcgtatacatagactgct
gaagaacagaactaccagccatggtcgtgtgggtggtacagcggcagtatatacagctgctatcttggaatatttgactgctgaggttcttgaattggct
ggaaatgcttccaaagatcttaaggtgaagcgtatcagcccacgtcacttgcagcttgccatcagaggtgatgaagaattggatgctcttataaaagcaa
ccattgctggcggtggagtcattccacacatacacaagtcactcattggaaagaaaggacagcagaagactgtttagtcaatgtcagaaacccaagaact
cgtcagacttgatcagccttctttctgaagaaaagactggttttcacactcatccaagactgagattcccatttcaaagcagatgcttggtggttttaat
gtgcccatggctttccaagaagccagttctgatggactctggttttacatacaggttttttttatgcaagttgttggggagatgtataaacctggaaaca
gcttgatgcttgatttcgtttagcaaaatgaattttaataaaatggtttggcctttaatggggtattgtcatgggacattgccatttttaatttttctaa
gccctggttaaacctttaaaaaagaaacccactcttgttggccaattacctaaacaatttttgtgaacatt
3' EST: CF784863
[+]tttttttttttttttacaaccacaaaatcgcagtattttaataccatgatttaacacaacaggagaaacatttcaaaatctagtattgcacagatggtct
atttcagcttaacagctagaaataaaatgcaattacatgacatacccattaagccaaacatttattaaattcatttactaaacaaatcaagcatcagctg
ttccatgtttatacatctcccaacaacttgcataaaaaaaacctgtatgtaaaaccagagtccatcagaactggcttcttggaagccatgggcacattaa
aaccaccaagcatctgctttgaaatgggaatctcagtcttggatgagtgtgaaaaccagtcttttcttcagaaagaaggctgatcaagtctgacgagttc
ttgggtttctgacattgactaaacagtcttctgctgtcctttctttccaatgagtgacttgtgtatgtgtggaatgactccaccgccagcaatggttgct
tttataagagcatccaattcttcatcacctctgatggcaagctgcaagtgacgtgggctgatacgcttcaccttaagatctttggaagcatttccagcca
attcaagaacctcagcagtcaaatattccaagatagcagctgtatatactgccgctgtaccacccacacgaccatgggctggtagttctgttcttcagca
gtctatgtatacgaccaactggaaactgcaatccagctctgggaggatcggggtgatagaggtagccttttgcttttccggtatctttgccagccttgcc
acca
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: