XB-CLONE-4351471
Clone Name: IMAGE:7027021
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Gene:
p3h1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784871 [+]
taattaatcccccccccccccggctggcgaagtggtgctaaagttgttgcccctcacagatatggcgctgtccttgctgctctccgggacagttttgttc
cttctcagcgctgaggtgctcgccgctcggctggaaccctatgactctctgtatgatagcggcgtgcaggcttactatcggggggactggcaccccgtta
tcctctttatggagaaggcgctgaggaaccgtgagctcctgcggcgccaccacatcagctgtcgcctgcactgtgccaaccgcaccggggggttcagcga
tctgccccttcactggggacccgtgcaagacctgacattgttccagaggctgctggaccgagctgactgcctgaagcggtgcgaagaggagaacatcggg
gcgccttctgacttctacatagccagccaggagctggagctggagttcagcaagcgcagcccgtacaactacctccaggtggcttattttaagatccata
aaattgacaaagccgtggctgctgcatacaccttttacatggcaaaccccgaacacatggaaatgagacagaacttggagtattacaaaaatgatgggaa
ggtggtcagggaatctgactttcagggaatctggaaagcaaaaacccccccattgcaccgaatttccggctttggggagttgagttaatttatacccgaa
tggagggaacacgggcgcccttttctatttggccaatttttttgaaaaagggcgccttgggaagggaaataatttttgtgaaaacctgtaaatcttttgg
aaatggttctcgagaaaacaaaggtgtggtagaaaaggggggcccccctcgctggggattttatttaaagggggt
3' EST: CF784870 [+]tttttttttttttttctcaaacattgccagtttttatttatatgagccacagtttcaagtcataaaattgcacattgaccgatttctctcaatcattccg
caaccacataaacatacaaaacatgtggaacagtgtaacatgaaaaactcacacttggttgttttgcaaacacaggctattctgcaagaccggctccaaa
taaataccccacccggtgtaggtccctaaatttattaccctggttcactgaaaaatacagtttgaactgaaatatcttctggcaaacaaaaaaccttccg
gggacccccaaagaaattttttggcccctttaaaaaatttcatttttaaaaaaataaattttttggaagccccccaaattcgggcccaattttttttttt
cccaaaaggggttccccaaatttttaaaaaaagttttccaaaaaaaccccccattttttccccccccgggaaaaagggggtttttccaggggggggaaaa
aaaaaaaatttttgggggggtatttttttcccccttttttaaaaaaaagtgggggttccccccccccccttttttcttttggggttaaaaaaaacaaaaa
aagggtgggggaaaaaaaacaccccccccgggggcggcgaaaaaaacccggggggaaaaaattattttttttttccccccccccctttggggggggggga
ataccccccccccctccctttttattttttttccccccacacgggggggggaaaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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