XB-CLONE-4351465
Clone Name: IMAGE:7027018
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Gene:
ptprs
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784875
[+]
taattaatcccccccccccccggattactcgatattggatcagggaaggtccaagcaactggcttagcagaaccactgaggatctaaattgcctacagag
tggatcactgttggaattgggaaatctgctacacccttggaggaaagggtaccaagtgcctgcgcctattgggagaaggagtccctgcccatctctttgg
gtgcccaggagtgggagttggtaagagattcctgtcccagttaccccccatgcagcctgagctgtaagacaactgttgtatctctttccctgaagggatt
gctaccctgtgactgaggggggctgttggattgaggactgcccgctccgctggccaggaaggactgagttggattgtgggggggcgtttaacccctgtgt
tgccggacattttgtggatattctttaagtgtgggacattccatcccttgttggaaagccattggctgcctacctgttggggtttaccagtgtggaattg
ctgtgttgttatgcattatactgtttaattaaaccagtaaaaccaattattggagttctgtggctttgccatatcctggcatcacaattgcctctgtgaa
tatttcagatatatgtaaccaaaaatcttgacattttggacattggtactaaatacactgctcaaaaaaattaagggaacacttaaacaacacaatgtaa
caccaagtcaatcacacttctgtgtctgggagacgccgtggaagaacgatctgcttgcctgaaacatcctc
3' EST: CF784874
[+]tttttttttttttttcttttttttttttttttttttttatcaatggaaatcaaatttattaacccatggaggtctggatctggagtcacactcaaaatta
aagtggaaaaagacaccacgggctgatccaactttgatatactgtccttaaaacaagtcaaaaagaggctccgtagtgtgtgtggcctccacacatccct
cctcagggatatcgtcccagacctggactaaagcatccaccaactcctggacagtctgtggtgcaacgtggcgttggtggatggagcaaaacatgatgtc
ccagatgtactcaattggattcaggtctggggaacgggcaggccagtccatagtatcaatgccttcatcttgcaggaactgctgacacactccagccaca
aaaggtctagcattgtcttgcattaggagaaactcagggccaaccgcaccaggggtctgaggatctcatcttggtacctaatggcagtgatgctacctct
ggcgagcacatggagggctgtgcggccccccatagaaatgccaccccacaccattactgacctactgccaaaccggtcatgctggaagatgtttcaggca
gcagatcgtttctcacggggtctcagacacaaaaagtgtgattgacttgggtgttacctttgtgttgttttaagtgttccctttaaatttttttgagcca
gtgtatttaggttcccaatggtcccaaaatgtcaaagaattttttggttaacctatttatcttgaaaaataattccacaaaagggccatttgttggaatg
cccggggatatttgggcaaaaaccccaccagaaaccccccacataaaattgggttttttaccggggggttttaatattaaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: