XB-CLONE-4351430
Clone Name: IMAGE:7027005
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Gene:
XB5768883
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784897 [+]
taattaatccccccccccgctagcccagccctgctgaggagtggagatctcccagccctagttctgccgcatctctcactccaagacccaaatacaagaa
tcaaacatgggagcaaaatttagtaaaaagaaaaagtcatattgtcttggtgctggtaaagatggagagtcaactgaaacaactgaagtggaacaaaaag
aaacagagactcaaagtgggcaaaaagatgcagttgtaccgaaggaagaaacacctcagcctgataaaactgtggccaacggtacaagtgaggaacaaaa
tcctcaggcggaaaagcaattagatcttgaaacaaaaccagatgaacccaccaaagatgatcaaaatgcaggccccgtatcagaaaacaatctagaatca
aataaagccgaaactgatgaaacgcctcaagaaaaagcagaaagcaattgcttaagtgcagttcaggaacagtcaagtgaagtggcaaaaggggacagta
gtagcaaggcgcaaacagcaccaccatctcaggagccagagaaaactggagaggagcagctgaacaaggatttggtttcgtccagtgatcctccatgtgc
tgctgtagcagagccagaagtgatacaaagcgttagtgtagagcagcaggtgactgtgcagcaagttgaacacttacaagaagctacagtgaaaggaggc
agctgaaggaatttcctccctgcatcaaaacttagctgctgaaggaagaaaacccaactgggtcactggaacatttcccatgtcctgaaaaaataaaggt
cccccttggcccgggaggggggtttccc
3' EST: CF784896 [+]tttttttttttttttgaatatgatttttgtttatttttggaaaacattgtgtagtttcatgctttaaagacaattgaatctgaacattggggctttaaac
tcaatgtaccttgccaatgttgatgctaattgttcttggagcttctttaaaacaggagacattttatggaaaacaaattaagaattgttctgttcagtga
aaagttagaaaacattaatataaataggacttttattctggaaatcacactgcatgaggctctttgccttgcaagtgtttacatttgtatgaggcatttg
gtcactgagaatgtatacagtgcatcaaaacagggaaattttggcttcttccacctttgataaaggggaaaaacacaaaacccacatgcgcttattcatt
acacacttcaatttcatggttttcattctgcccgtttagatcactaatagaatggttagaaccattttctacattttctttcaccttaatttcctctttg
cttggcaggccattaagggtcacatgcttgtctactatctgaacatcagcaagaattttctcatgtacagattcttcagctagtgttgcattgttttcta
ttgtatcttctgtgaggggctggggagctgaaccactaatatcttgtttatccactggacttgaggaaatatgattgctatctctttgttcttgatttan
ggtttcctgaaaacatctaccactgccttgctctcttcctcacacttttctcctgctctaatgaatgtttgtcaaagctttggtcttcggaatctagatc
attggcattangctcccaagggactgagan
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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