XB-CLONE-4351425
Clone Name: IMAGE:7027003
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Gene:
hnrnpa0
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784900 [+]
taattaatcccccccccccccgaaggcttttgtgacgtaagaaagaagagcagctgctacttccctctcctcctttcgccaccagataaaacggagagag
agcgcctgagcgcgacgatctgtgcttgcgtttcaatgagtaccggcgatatggatacgacgcaactctgcaaactgtttatcggggggcttaacgtgca
gaccaccgaagagggcctacgccagcattttgagacctacggtcaactgacggactgcgtggtggttatcaacccacagaccaagcggtctcgatgtttc
ggtttcgtaacctactcgagtgccgcggaggccgacgcggcggtggaggcctcgccccacgtggttgacggtaacaacgttgagctgaagcgggccgtct
ccagggaagattccgccaagccgggggcacacgctaaagtgaagaagctctttgttggcggcctgaaagaggacgtcggcgagagtgacttgctcgaaca
cttcgcccagtttggcgccgtggaaaaagtggagatcattgccgataaagtgtccggcaagaaacgcggcttcggctttgtgtactttaccaaccacgat
tccgccgacaaggccgcggtggtcaagttccactccatcaacgggcaccgcgtcgaggtaaagaaggccgtgcccaaagaggaactttgccagggttcca
atagatccttccncggcggcaggggcggggaaaggccnaaagtgggcgggcggaacccccccggggactacaatgggcattccctgggaaagtggcgggc
3' EST: CF784899 [+]tttttttttttttttgggtaataaaccgatttatttaaaaagtaaaaacaaaaaagggtacagggggtactgtttgcttgcagcagcccaacaggctggc
caaaaacaaaaaaccagggtgggtgtgggagaatacggcttccttaactgccattgtcagtttcccgctctagatctcaggttacaatagcataaaaaca
atcagagcccaaaacacacatgaaaatatgaaagaagttcgagtttatcggtgtgtttgtgtggattatttccatctctcactgcgccaaagttattaaa
cctttcagaaggagccgccgccataggagctgccgacgccataacctcctcctccgccgccgccgctataacctccgcggtaggggcctgacgtgttggg
ccggttccagctgccgcttcccgctcccttcatgggcccgtacgaggactggtgctggctgtagctcccgaaactgttgtagccattgccgtagtcgctc
tgctcgccatagccgcctccgtacgagccgtagccctggttgccgcccccatagccgccgtagcccccaccgaaagcagctttgccgccacttccaggga
tgccattgtagtcccggggcgctccgccgccacctcggcctctcccgcccctgccgcncgcggaaggatctattggaacccctggcaaagttcctccctt
gggcacggccttcttttacctcgacgcggtgccccgttgatggagtggnaaacttgaccaccngcggccttgtttcggcggaatccgtggttgggtaaaa
gtacaccaaagcccgaaagcccgcgttttctttgc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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