XB-CLONE-4351419
Clone Name: IMAGE:7027001
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Gene:
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784904 [+]
taattaatcccccccccccccggtcagaagtccagcacactcttggagagaggattatgacaagaaagtgaaatgccaccaagaaacaaggcaaaaacgt
ttactggcagcaacacacctaagagccgaaattctgcatctaaacctaagaaaaatagaaggtctaagcatagtgcaaaggccagacatgccaatgaaga
acatagcactcggtgcaacagccccttggtgtataatcctgaggcaattgttgaagaactaatggccaaataccccccaaatcagcatgaccctggtcta
aaggagctctatgaatatgaaaacaatagacagagggaaccgggactccctgtaaggatgggcctttccaataaaatcagtcgctttgagatcccagtgg
atataaaaatactagaagatctgactgcccaggattacctcagaacttactgccgagtgtgtaaaagaaggcagctttattataagaaattctttgataa
atttgacaaagacagagatggtgccctctcctcgcaggcaacagaggatgccttgaaacatctatactttaatgaaataagctccagtcaagtgaaagat
ctcatgaagtttgataaaagccaaagaggacaccaagttgggacttgtatgctttttcaggctctcctgttgtgcccctatcagaaacgcccttttccta
aactaccttatataacaaaagaaatacccgggcagttgcgaaccaattaaaaaaggcaaatggctttggaaggcaaaccaagaacttccttcctgggaaa
gtgaagttttggaaaaaatatgtccaggggcctggttaaacaattttgaatttaaagaaacctttttttaaagc
3' EST: CF784903 [+]tttttttttttttttagatttgccgtgcctttaattacaggaaaagagtaatggagaatgttctggaaccaagtagtattgtgtgacacacaagcattct
gattcactgccttagctaccatctgccttctggcgggcacaatagtaccccaaagagtctctttaaagtctcatcaatgttacagcctgcatatttccaa
ctcattccagagaagtctgctgcctcaagccactgcctcttattggtcgcactgcccgtatcttctgttactaatgtagtatagaaaaggcgctctgata
gggcacacagagagctgaaaagcatacagtccaacttggtgtcctctttggcttttatcaaactcatgagatctttcacttgactggagcttatttcatt
aaagtatagatgtttcaaggcatcctctgttgcctgcgaggagagggcaccatctctgtctttgtcaaatttatcaaagaatttcttataataaagctgc
cttcttttacacactcggcagtaagttctgaggtaatcctgggcagtcagatcttctagtatttttatatccactgggatctcaaagcgactgattttat
tggaaaggcccatccttacagggagtcccggttccctctgtctattgttttcatattcatagagctcctttagaccagggtcatgctgatttggggggta
tttggccattagttcttcaacaattgcctcangattatacaccaaggggctgttgcaccgagtgctatgttcttcattggcatgtctggcctttgcacta
tgcttagaccttctatttttn
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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