XB-CLONE-4351416
Clone Name: IMAGE:7027000
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Gene:
pdlim1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784906
[+]
taattaatcccccccccccccagggaaagagtcccgtgtggaacatccacagcccgagttgggagcggagaaccatagtggcacaaagctgggcacaaca
tggctactaaacgtatcgttatgactgcgcctccaccctggggattccggctgattggagggaaagatttcgaccagcccttaactatttccagggtaat
gccgggtagctaagttgcagttgccgatctatgtattggagatgtagtcatttctattgatggggaaaacacagacggcatgactcatttagaagcacag
aataaaattaaaggctgtgaagatgaactaattctcacaataaacagggttgaatcgaaaatatggtctcctcttgtgtccgaatatggaaagccatatc
cttacaaaatgaatttagcaagtaacccaccggaatttaaacaaataggaagttgcactatagtctaagtgctatgccgttccactggctgcctttcttt
caagcaccacccaaaagtggatcacctgccccattacaaacaaccccatgaacgggcctcactatcctttccgtaaaaccatatgagaaactttgcagag
attggcccctgggaaggacccaaattccgagactcccctcaaatgtcccccaaagtaaccaaattcaaagagccttcgggaaccccccccccagtcgtgg
gaatttcaaccccgaacaccttggagaaaaagaacacttgaaatgctttacaaaaaatcgtttggcacgaaaatatccaggcagtatttagtatataaac
accccgtcagagaaaatgacgagattccttttcttaggtccacggcaaggaaatatcgtgtgaaaattggtgttgaataagggtgaataaagcttgcctc
ggggctaccggagatcgtaaggggacccc
3' EST: CF784905
[+]tttttttttttttttcaagttatacaagtgtctcctgtattgtacaacaacatctaaagcaacaagacgaaacacacacaatggcagttagattgaaatg
attgttgtgtagaaacgtctcacacaatctcctaaaagcacttcctgcaaacagaagggacattcacatctcagcaaatagaagcttggcactgttctaa
aatggcgcagatgaatggtacttggcaagccggcgcggcggatagggaagagagttatttggggaacactgtgaccacgtcatatccctcgggcggtgtc
atgcgttcccgtgcgtgcttttcacagtacatcttgtcttctacaaagaagtggcccttttgcttgaggttcatcccacagtcggtgcaaacgtagcatt
cggggtgatgaggtttgtcccgaatcttcacaaacactcccacaattcctgagccacagtgaccacaaataggcagtttttggccactccccagagaggc
tgccactttagttgtgggtgcccttacactccggaaccctgacggcttttcccctttctcatcagtttccaagatttcctgcaggaccaagaaagaagcc
gactgcctagggggttcatctaattcttgattctcctgcagcatcttgtaaacttcagactctttatcaaggtgtcgagtatttgcccggctgtgttggt
tcccaaactttgcttggtacttggggcactggggagtcgatttgggtctccaagggcactattggaagttctctaaggttgtccgaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: