XB-CLONE-4351410
Clone Name: IMAGE:7026998
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Gene:
darmin
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784910
[+]
acaaggctcaaggagagatctgcactctggtggctttggagggacagtacatgaagctatgagtgatttgatatatttgctgaacacgctggcagatgga
aagggtcgcatccttgtcccagggatttatgaggctgtggcacctgtgggtgagaatgaaacagatttgtacaaaaatcttgaattccgtcaacaggaaa
tgcaagctgacacaggggtcacgcaattccttcatgatacaaaagaagatttacttatgcatagatggcgctatccttcactaacaatgcatgggattga
cggggctttctgtggaacaggaactaaaactgtaatccctgcaaaaataattgggaaagttctctatgcgtcaagttccttaacatggatccacttaatt
ctagaagcgaccagccaccagctgtgggaactttgggccgtgggccccaaacttgatttaattagcatcctttattattggctttacaaattaaattcaa
taagccatctccatattttccacttaaattaaaaagccattttttttttccaacaggaaattatcttaattttggttgcgctatttgatccccaaaaaca
tcctattcaaaatgggtaaatcctatgtatcttattagtgtcattgggagaatatcgggagaaaatttttttatttttctccgtgtcacggtccattcga
caacctcctctttggtgattcacgttttgtgtttagatgcttaaaccccctatatcttttgtggggaaaaatgacgtgtaaagggggaaaacatcccttt
tgtgggcaggtttaaaaatatggcgcccacaacccccttgttgttccaattggaaaaaaacgggtcaatgggaaaagtataagagaaatccacccctttg
aatcctttggagatgaggcgaaaaaatatgaagctacgttgtgtttaccacaaccttttcctaacanctcttactttgacatctgaacactaaaaaaaga
aatgtaacccccccccactataagaggagacctacctcacacgcacatataagataaatatgattcttatctaaaaaagtgtntaatagaggtgataaaa
gagtgccggagtggttcctcctctctctctccaaaatattatatatgttttgatattcactattcacacatagagngtgatcgcaaggctaacaaaacga
acttcctcccccacgacatgtgctctttcacccctccaagatatatttggcgcgatgaacaaagaattttttatctacggataggaatcgcttgtcatca
ctactcgttatccgattg
3' EST: CF784909
[+]tgttaggaacttgacgcatagagaactttccaattacttttgcagggattacagttttagttcctgttccacagaaagccccctcaatcccatgcattgt
tagtgaaggatagcgccatctatgcataagtaaatcttcttttgtatcatgaaggaattgcgtgacccctgtgtcagcttgcatttcctgttgactgaat
tcaagatttttgtacaaatctgtttcattctcacccacaggtgccacagcctcataaatccctgggacaaggatgcgaccctttccatctgccagcgtgt
tcagcaaatatatcaaatcactcatagcttcatgtactgtccctccaaagccaccagagtgcagatctctccttgagccttgtac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: