XB-CLONE-4351390
Clone Name: IMAGE:7026991
|
Gene:
krt18.1
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CF784923
[+]
taattaatccccccccccaccccgagccagaagttgtcccggctgagcatctccgcaccacttacctgcgcagccatgagttactcccgctccgtgtatt
cttcctcctctgtagtaggggggagtccctaccgctccctcagctccgcaccccgctttatacccagtagcagcgccgctagtgtccatgctggggcagg
gggctccggggcccggatctctgtatctagggtatcctcagtggggtcagggtttggcggtggctatagcggggtatctaatgtgaatctgatcggcggg
gggcagaatgagaaggagaccatgcaggacctgaatgaccgcctggcatcctatctggagcgggtccgcagcctggaggcagccaataagaaactggagg
tgcagatccgccagcacacggaaaagaaggggccctccaaggactggagtccgtactacaagactattgaggacttaaggaaacaggagtttgatagcac
attagagaactcccagctggtcctgcagattgacaacgcacgccttgcagctgacgacttcagagtcaagtatgaagcagagatggctatccgtatgtct
gtggaaggggacattaccgggttgcgcaagcttatagacgacaccaacgtcagccgaatgaacttggagaatgagattgagtccctgaaagaggagctta
tcttcctgaagaagaaccaccaggatgatgtgactgaactacaaggctcaagttgcaaaggtctgcctgtaaactgttggaaagtggaatgccc
3' EST: CF784922
[+]tttttttttttttttagaattctggaatgtttattaaatgaaatgcaatggacatgaaaatctgtttccaagagttaggacttgaggacttcagtatcat
tgctttccgaaaccactttcccatccacaatcctctgtgtggtcgtgatgactttctttaccgtctgtgtggttaccgttggcacagcatcttggaggtc
aaaactgccgtctccttccaggagccttctgtaggtatggatttctgcttccagcttcatctttgtgttaagaagagcctggtactcctgttgctgtctc
tggcagtcactgcgaacttgtaccagctctgcctccattgccatggcagtacccccaagcatctccagctccatcgcataacgtgcttctgtgtcgtgga
gtgtgccctctaagcttgctttctgattgcgcagagactccagctctatctctagggactgcacagtgcggcgcagatcagttacggaactctttgcaga
gtgcagcgcttctgtatctaggttaacttgcactgtgtgctcatccaccttagactgataccacttctcaacatcatcgcggttcttttgtgccagtcca
tcatattgtgctcgaagttctgtcatgatcttgccaaggtcttgtgatttgggggcatccacctccacagttacagcagaccttgcaacctgagcctgta
gttcagtcacatcatccctggtggttcttcttcaggaagattagctcctccttcagggactcaatctcattctccaagttcattcngcctgacgtttggg
tgtcgtctataagc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: