XB-CLONE-4351384
Clone Name: IMAGE:7026989
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Gene:
tubb4b
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784927 [+]
taattaatcccccccccccccgagcgctccaaggtaaccggttcagttcggagtttagtacagagcaaccaactacagtcgccatgagggaaatcgtgca
tctgcaggccggccagtgcggcaaccagatcggagccaagttctgggaagtgatcagcgatgaacatggaattgacccaaccggtacctaccacggggac
agcgacctgcagctggagagaataaacgtctattacaatgaggcgacaggtggcaagtacgtcccccgcgctgtgttggtcgatcttgaacccggaacca
tggactccgtgcgctcagggccatttgggcagatctttaggcctgacaactttgtgtttggtcaaagcggtgctggaaacaactgggccaagggccacta
cacagagggagcagaactggtggactcagtgttggacgttgtgaggaaagaagcagaaagctgcgactgtctccagggcttccagctgacccactccctg
gggggcggcaccggctcgggcatgggcaccctgctcatcagcaaaatcagggaagagtaccccgacagaatcatgaacactttcagcgtggtgccctccc
ccaaagtatctgacacagtagtagagccgtacaacgccaccctgtccgtgcatcagctggtagagaacacagacgaagacctactgtataagataacgag
gcccctgtacgaaatcttgctttagaacccctaaaaattcaccaccccccaacctacggggggactggaaaccacctttgggtcctccaccccacccttg
aagtggggggttaaacccacccttgccctggct
3' EST: CF784926 [+]tttttttttttttttacgaaaaatgcttttaatgaacggaacgttctgtacaactggtaacaatgaaaccggagagagaaggaaatgcaggaaacaaata
aaggaacgaacgtgagcagaaacagttgaataatttacagtagagatcagaagcttatgcattttcctcctcttccccttcctcgaactcgccctcctcc
tcggccgtggcatcctggtactgctggtactctgacaccaggtcgttcatgttgctctcggcctcggtgaactccatctcgtccatgccctcgcccgtgt
accagtgcaggaaggcctttcggcggaacatggcggtgaactgctcggagatgcgcttgaacagctcctggatggccgtgctgttgccgatgaaggtggc
ggacattttgaggcctcggggcgggatgtcgcagacggcggtcttcacgttgttggggatccattcgacgaagtagctgctgttctcgttctgcacgttc
agcatctgctcgtccacctccttcatggacatgcggcctcggaagacggcggccacggtcaggtagcggccgtgacgggggtcgcaggcggccatcatgt
tcttggcgtcgaacatctgctgggtgagctctggcacggttaaggcgcggtactgctggctgcctctgctggtgaggggggcaaagccgggcatgaagaa
gtgcagacggnggaaaggcaccatgttgacggccagtttgcgcaggtcggcgttcagctgcccagggaacccgcagggcagtgggtaccccactcatggt
ggctgagaccaagtggttcaggtccgc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
Suppliers: