XB-CLONE-4351375
Clone Name: IMAGE:7026985
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Gene:
eef1a1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784933 [+]
taattaatcccccccccccccgcttcctcaccgggtctgcggcgagttttaagtgtccaccgccaaacatctaacaatccacaatgggaaaggaaaagac
tcacatcaacatcgtcgtcattggacacgtagattctggaaagtccacaacaaccggacatcttatctacaaatgtggtggtatcgacaagagaaccatc
gaaaagtttgagaaggaagctgctgagatgggcaagggctccttcaagtacgcctgggtcttggacaaactgaaggccgagcgtgaacgtggtattacca
ttgacatctccctgtggaaatttgagaccagcaccaattatgttactatcattgatgcctccagtacacagagacttcatcaagaacatgatcactggaa
cctctcacgttgaccgcgcctgtcctgattgctgctgtctgggtgttggcgtaatttgaaagctggcacctttaaatacgtggacaaaatctcgagatgt
attgccccatcttttgcttttaaatactgcggtaaataagatctacaacctgaatcggttaggcctgttccctccccaaaatagttattttcaggttgca
atcacctttttttacccgttcgttaaatgcctccattattgggagggaaaatatcttaccaatagcgaaagtttatgtcacccacttcctttctatataa
aaaaaatgtgtacaacagctgcgttacaaatataaaacgcccctttttcccccccccttttttttcgagatggcaaccaaatttttcctacccttgtctt
ttttattttttccccccctttattctccccctctttgttacaaccgttggccggaaaaaactctcccaaatacattgaggtgatcattggtgatataccg
gactatatattctgccttttttcccccccacgggatatatatccatacccgctc
3' EST: CF784932 [+]tttttttttttttttctatcttttaacttttttttaatggtctcaatttggtgacagattttggtcaagttgcttccatttagaaattactggatttaaa
acactaataacttaaaaactgccacaaacaaatgtgtccacgagcatttttttccttctgaagcttttgcgatgcattgtaatcattaaccagtctttga
ctattaaactctgatggccaattgatagaaagagttctgagaccaatcctccaccactggttatgactgaggtgagagatgctggaaatattcatttgcc
tttctgtgctttctgagcagactttgtgacttttgccagctccagcagcccttttttaacgaaccgcccttgatgacttcctaaagcaacagtcttgccc
ccattgtcccgggaccggccaaaaccgacccaaaaaggggggggtaatcccaaaaaaaacccttcccccccgcacaaaaagggctttttcccggggggaa
attcagggtccaaaaaaaagggggggggggctatttcccccaaaaaatttttttgggggaaaaatcttgggggggaatggggcctctcctccccaaattt
tttttttttctccccaaaaaaaaggggggggaaaaaaaaaaaaaacatttttttttttccctttttttgagccccccccacaaaaaaaaaaaattttttt
tcaaaacaaaaaaatattatgggtagccccctctttgtgggtgtggtgggtaaattctccccacaaaaaacagaggggggggggggtgggatttattctt
ccctccccgccccccc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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