XB-CLONE-4351372
Clone Name: IMAGE:7026984
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Gene:
ctsv
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784935
[+]
taattaatcccccccccccccgctgcaatttccagcaaaacagcctgagaggatacatcatggcactctatctaggtattgctgctatctgcttaaccac
agtgtttgctgctccaacaacagatccagctctggataatcattggaatctctggaagaactggcataaaaagtcctatgcaccaaaagaagaaggatgg
aggcgagtgttatgggagaaaaacttaagaatgattgaattccacaaccttgaacactctctaggaaaacattctcatagcctgggaatgaatcagtttg
gtgacatgaccaatgaagaatttagacagctgatgaatggctacaaaaaccaaaagaagataagaggttcaactttccttgctcccaataactttgaatc
acccaagtctgtggactggcgcaaaaaaggctatgtaacaccagttaaagaccagggacaatgtgggtcatgctgggcattcagtacaactggtgcgctt
gaaggtcagcactacaggaatactggtaaaatgatatctctgagtgagcaaaatcttgttgactgctccagagcccaaggaaaccagggatgcaatggtg
gcttaatggatcaagcctttcagtatgttaaggataatggaggcatagattctgaagactcctacccatacacggctaaagatgaccaggaatgtcacta
tgatccgaactacaattcagccaatgacactggttttgttgacgttacatctggaaatgagaaagatctcatgaatgcagt
3' EST: CF784934
[+]ttttttttttttttaaagttgaattaacaagtttttattttacattataaaagcttaaatcacataaaacacccattaaaatcaacatgtaaacaatact
aaaacaacccagcttcaatgtgggatgttaacgtccaacacaaatttaaaagacagccttgagcagcaattccatcaggtaaaaacagtccagtacttga
taaaagtggagtttcacaatatgtgcacataaagttccaaggtcaaaaaggtccatatgcaatggtttattttaaaccagaggataactggcagctgttg
ctattccacagtggttgtgtctatccttggcaatgtatatgtatccatcgttgccccatttctcgctccagctgttctttacaatccagtatttcttgcc
atcctcatcttcaccctcaaatccatagcctacaacgagtacaccatgatccaggtcttcactgctgcattcaggctcataataaatcccagatttataa
aactgaaatgactgatgtcctgcatcaactgcaacagagactggtcccactgaagctactgcattcatgagatctttctcacttccagatgtaacgtcaa
caaaaccagtgtcattggctgaattgtagttcggatcatagtgacattcctggtcatccttagccgtgtatgggtaggagtcttcagaatctatgcctcc
attatccttaacatactgaaaggcttgatccattaagccaccattgcatccctggtttcctttgggctctggaagcagtcaacaaggattttgctcactc
cagagatatcatt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: