XB-CLONE-4351369
Clone Name: IMAGE:7026983
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Gene:
hspa8
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784937
[+]
taattaatccccccccccccccccccgctcattgcaagctttacaagctcggagacggcgagcagctcatttaaaaccagtcgtccatatcacattactg
agagcaacaatgtctaagggaccagcagttggaattgatcttggcaccacttactcatgtgtgggtgtcttccagcatggcaaagtggaaatcattgcca
atgaccaaggaaaccggaccacacctagttatgtggcttttacagatactgagagattgattggagatgcagctaaaaatcaagttgccatgaaccccac
aaacacagtttttgatgccaagagactcattggccgcagatttgaagatgcagttgttcagtctgacatgaagcactggcctttcaatgttatcagtgac
agtggaagaccaaaggtccaggtagaatatacagcaaagaccaaatcttttctatccggaggaaatatcacctatgggcctcagtttagatgacggaaat
agccgaaccttaccctcggcaagacgtgcaccaaatgctgtaatttactgtcccattattaatttcatggactccctcgccacaggcctaacaaaggccg
ccgggttactaatatctggggccgaaatggaccggccaaaatctttaaaggaagccaacttgggtgccggccctttggccttaaggggactttaaataaa
caccgggtttccgacgccccatattgaacgccgtggcctgaatttttttaaaacccttggggtaggggggtgaaccctttctaaattctcccccctgttt
tataacaacccttgtaacccaagaggaaacatctttctaaaactataagagattctaaacatcgcttgagttcggcgacccttccattttttagcggggt
gggtgaaacaacaccacatcttgcaatatatttatctcgtagtgtgggtgagaa
3' EST: CF784936
[+]ccaaccaatacaatatcgtgtatttgtgacttgtcaagcttagcatcacgaagtgctttttccacagggtcaagggtacctctaaagagatcagcattaa
gttcttcaaaacgtgctctggtgattgaggtatagaagtcgattccttcatagagagaatcaatttcaatgctagcctgagtgctggaggagagcgtacg
ctttgcacgctcacaagcagtacggagacggcgaacagctctcttgttgtcaatgatgtccttcttgtgctttctcttgaattctgcaacaaagtgattc
accatacgattatcaaagtcttcaccacccaaatgagtgtcaccagcggtagactttacttcaaagatgccatcttcaatggttagaatggagacatcaa
aggtgccaccaccaagatcaaagatcagcacgtttctttctgctccaaccttcttatcaagtccataagcaatggcagcagcagttggctcattaatgat
tcgcagtacattcagcccagatatagtaccagcatcctttgtagcctgtcgctgggagtcattaaaatatgctgggacagtaattacagcatttgttaca
gtcttgcccaggtatgcttcaactatttccttcatcttaactaagaccatagatgatatttcctctggatagaaagatttggtctctgctttatattcta
ccctggacttttgggtcttccactgtcactgataacatttgaaaagcccagtgctttcatgtcagaactgaaccact
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: