XB-CLONE-4351347
Clone Name: IMAGE:7026974
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Gene:
tpt1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784950
[+]
taattaatcccccccccccgccttttctttccccaccctccgccggcactgatcctccctgtgtgctccggcactacaacacccaggccgccaacatgat
catctacaaggacaccgtcactgaggatgaaatgttctccgatatctacaagatcgccgaaacccccgacggcatgtgttttgaagtggaagggaagatc
atccaaagagtagaaggtgcaattgatgatgcgcttattggtggaaatgcttcagctgagtgccaggaggaggacatcggtggtgcaacaacagtcagcg
gggtggacatagtgattaatcacaaacttcaagaaactggtttcaccaaggactcttacaagaattacattaaggattatgtaaaactagtcaaggccaa
acttgaggagactgatcccgacagagtaaaacccttcatgaaaggaattcaagatagagtaaaactcatacttggaaacttcaaaaactatcagttttac
actggagaaagaatgaacccagatggtatggtggctctgctagattaccgtgaggatggtgtaacaccttttatgattttctttaaggatggcttgatca
gtgagaaatgttaacattcaatttggttcttccatcttggctgctgcttttcatccatgaacaccangtctaagcaagatcttactgactcctaaaggac
tgatgatacattgggcttgtttcttcatggccctaaattattcaagtggaggcttttttgtttttttttaattgaaatccttttgtcatttagagtaaat
aaaagcccata
3' EST: CF784949
[+]tttttttttttttttttttttttttcattgtttaatcttgtttttatttaggatcagtaagaaaaaaagggaaagatcttctgtgatggtagagcctgtt
aacaggcaacagtcaatgttccaaatccaaacatgttcttgtgcaaacaacctgcagtgtaaccaaccccttcacagccaaaggtatttgcaacactgtt
gaaaggccttctggcagtggaggagtctaaaaacctgaagccgaaaggcaaattgaatactttggctgtttccttaagtgtgcagattgtccacaaactg
aaggtccgcttcttacaaaatgttctggcagtcaatctgataagtcaaagcttttgcgcaacaaatcttaaatgcacaagacccagtcttcaaaagtaat
tgcagatcaaaccaaaaggcaatgtctacagtggaaaattatgggcttttatttactctaaatgacaaaaggatttcaattaaaaaaaaacaaaaaagcc
tccacttgaataatttagggccatgaagaaacaagcccaatgtatcatcagtcctttaggagtcaataagatcttgcttagacctggtgttcatggatga
aaagcagcagccaagatggaagaaccaaattgaatgttaacatttctcactgatcaagccatccttaaagaaatcataaaaggtgttacaccatcctcac
ggtaatctagcaaagccaccataccatctgggttcattctttctccagtgtaaaactgatagttttttgagttttcaaagtatgaattttactctatctt
gaattcctttcagggaagggttttactct
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: