XB-CLONE-4351344
Clone Name: IMAGE:7026973
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Gene:
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784952
[+]
taattaatcccccccccccccgatccttgctctctgctttgtcctagctgacttgtcggagaatatctaccaataaacagccatcatgacttcctatagg
tccagctctgcttcatactattcaagctcatccggcaaaggtggctttggaggcagcagctatggaggcagcagcttggcaggcagcggctttggaggag
gctttggcagcggtgcaggctctggcttctcatcctctgggggtaactttgccatggcggcggcagccagctccagcttaagcaacttcggtggaaatga
aaagcacgccatgcagaacctcaatgaccgccttgcttcctacctggagaaggtcagagccctggaagcctccaatgccgacctggaagggaagatccgc
acttggcacgagaagcaaacaggcaccggccttggagctgggtctaaagactacagcaaatattttgagatcatcagtgagctgaggggcaagatccacg
gtgcaactgttgataacgctacagtgaccctgcaaatcgacaacgccagactggctgctgacgacttcagacttaagtttgagaatgagctggctcttcg
ccagagcgtggaggctgatatcgctggcctgcgtagagtcctggatgagctgaccctggctagaggtgacctggagatgcagattgagagcttgactgaa
gagctgggctacctgaaaaagaaccttgaggaggaaaatgatgcattgccaagagccagaagcgccggaaaaaatgaattgtttgaagatggacgccggc
ccc
3' EST: CF784951
[+]ttttttttttttttagagttaaagatcttttattgctttatgagacatttccataatcatttcttctttcttacgcaggtcttctctcttgtttcagggg
aaatttctgttcatttgttcctttatctttcccgactgcccttggatttatgacactgttgaagaaacaaccttcccatctaccacctcttcgacaatgg
tttttactgttctggttctggttgttgttgttgatgatgattctgagctggactccagggaacttgcctttgacactgtggtcatctgtcccagttcccc
atccagcaggcgcctgtatgtttcaatctccatctccagcctggtcttaatgtcaaggagctgcttgtactccatgttctgatgttccatgtcggacctg
atctgcagaagctgttcctctaggctgctgatagtgttctgaatctgtagaagttgcgctgaatagaagccttgtaattcattgaggttgccttcaactg
attgtttcattgccagctgggattgcagctcaatttccaagctctgaagtgaccgtttcagctcagtgatttcgctcttgctggcctgaacttgttcaac
cccaactgaaatctctttcttcagctcaccgctcttctgggtgaaccacaactctgcatccctgcggttcttctcagccagtatttcatagtcagctctc
atgttattcagaatgttggtcagatccacgcctggagccgcgtccatctcaacactcacttttccgggcgctctggctcttgg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: