XB-CLONE-4351331
Clone Name: IMAGE:7026968
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Gene:
pnrc2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC080143
[+]
CCCCCGGTGAGTTGCGATTTGGAGGCGAAGGGTGGAAGTAGGAGGAAAGATTCGCAAGTCTGAGAGTTTTGCGGTTTATCTGAATTTTGCTTCGTTTCTT
CACTCTCCGCTACGTTCAGTGGGGAGAGGAGACATTTCTCGAAAAGAAGTCTCGTTTAGAGGACTTCTTCCATTGTAATCCCCGGTTGCCTTGTGCCCAG
GGGGATTCGATGTTTGTTTTGGATCTTGATAATCACATGCTTAAGAAAACTAGCAGATTAAAGAAGGCAACACAAAGAGGGGAGCGAGCCTTGTTAGTTC
GGAGACCTGGAAAGGCAGAGAAGGGGTGTGAGGAATCAGTGGGAAGGTGACATAAAAGTTGGAGATGGGTGGAGGCGAGAGATTTAACATTCCTGGTCAG
CATAGAAATAACCTTGGCAAACAGATTAACCGTCAGAAGTTGTTAGAGCGCAACAATCAAAAGATGAACACGTCTCTCAGTAAGGACAGGGTCCGTGGCT
GTGGGACATCTCTTGCATGGCAGGCCATGCAGAATGGAGTTAACAACAATAACACTCGTTCCTCGAATCAAAACTGGAGTGCTGGGTTTCCAGCCTCCAA
GAACTTCTTCACCGATCAAGACAACCAGAATTACGCTGGAGCCAAATTTAGTGAACCACCATCACCGAGTGTACTTCCCAAGCCACCAAGCCACTGGGTG
CTCCTTTCCTGCAGTCCAGCTGAGAAAGAGCTGATGTCCTTTCAGCTGAAAACCTTGCTAAAGGTCCAGGCCTAATTTGGACCTACCAAAACCATCAATG
ATAAATAAATGCCTATGGGACACTTACAGGGTTCTAGGTTGAAGAATGCCAAACAGTGTGTTAAACATGAGATCTTACTTGACTATGCCAGCATGGAATG
GATCGTTTTTTTTCCTAAAATCCCTCAGGAGTTAAACTGAATGGTGGGTGAAGCTTCCGTGAAGAGGACTCCCGCATACGAAGCACTTTCTCCACAGCAT
AGCTGGTGCTTGATGCACTGAATTTACAACTTGCACCCAAATTAATGTTATACAACACTTTGCACTTGAAGTAAGCAGGTGAACAGCTTCAGGACTTGGC
TTTGGATTGTTTTCTTCTGCTAACTTTGAATGCTGTTCACCTACTCTTGTATATCTTTAATTTGCAGCGTGTGCTGTGGTAGCTCTTAGTTATGTCATCA
TTTTATTAAGTTAATGGAGTCTGACATTGTTTTAACCTGTGTATTATTTTAGTCTTCAGCCTGGCTGTATGGCCCATCTAGTTGGATCTTTGCAGTACTG
GGCTTACAAAATTATACAAATTGTTACAAACAAGTACATTCTTCCCTTAAGGCAGTGGGTTCAAGCACCCACTCCTGTGTCACAGCTTTGGGTAGACACT
TTAATCCATTACAAATATGTAGAGATATATATACCACCTTGAGGAGTATCCTCCAAGACCATGATCTTAGTATTTATGCCACCTGTAGAACTAAGCCTTG
TTCCCTCCTGGAGTGCTGCTCTTCAGTTTGGGAAAGTGTGCCTTGAGGTTTTAAGGGTAATTATTAAGTACAAATGGTTTTTCCCCATGCTGAAATGAAA
CATTCTTAATTGTGATTTAACATTTTGGACAGTTTATGATGTTTTAAGTCCTCCCCTAATGCGTTCCTTGTATTAATTAATAGTAGGCAAGAGTTAAAAT
TTTTGTATAAAAATGCCCAATATGTAAAGTTTTCCAAAGTAAGACAGGATATCACCAGGAGTGAAATGAGCATCATTTGGTTGAAAAAATGGTTTATACA
AAATAGTGAACTTTTTCCCCTACTCCTCCCTTTTCTCCCAGCAGAGTAAGTAAAAGGGAAGCAGATCCTGACCACAGGTCCCTTTTTATTTCAGGGTGCA
ACATCTGCACAGAAAAAATCCGGTTTGCAAATCATGCAGGCACTGCTTGTAGGGCAAGGCTGAGCATATAAAGAAAATAGTGAAAACTGTCCAAAATGCA
TTATATTGGGAAAAGTTTTTCTCAAGTACGGGGATCAGCTTTTTTTATTTTATTACAGATAGTACCCCTTTAAACAAAATTTTATGCACAGACTGATCCT
AATCTCTGAGGTATAGTGAATAAGATTACTTTAGATCTGCAAATTACATTTATTGTGCCAATGAGAGTATATTTGCACTGTAAGACTGCCAAATTTGAAA
TCCTAAACCTGTTGATTTTCGTCCAAAAAGTAGATTGCCCGAAGTATACCTTTTATTTACTAACATCTGTCAAGGCTGTAATTGAGAGAGAGAGAGCTGC
GTACGTATGTCTTGTTTACTATGGTGGGTGGACATGACTGCCTTTTGCGGTCACTTGTGCTTATTTAGCAACACTGGGCAAATTTGTATCTGGGCAGTAG
CCCACGGTTCCAATCCGTTTTTCTTTCGTTCTTCTACCTGCAGCTGGTGGGGAAAGAAAGCTCAAAACCTATTGGCTGCTATGAAATACTGTCCTGGTGA
AGCTTTGCCCAGTGTTGGCAGATAGGCCCCAGTTTATCTTGCTTTTAGGAAATGAAGCATGTTTTGTAGCCCGCAACAGATCTTAGAATGATTCTAGCCT
AATAATGAATAGCCCTGTTTTACACATCCCTTGATTGTTTAAAGCTCTTACTGTGAATTATACTTCAGGGTAATGGAATCAGTTGCAGGCTTTTTATATG
GTTAGTTCTAAAATGAGCACCCTTCAACTTGGCACCCTGTAATGTCTTCAGAACATATTATTCAATCTAGAACAACTAGGAGCCATGTATATAACTGTAA
AAGTTGTAGAATATGTAAATGAATGCAGGCAAACCTGCAAAGATAAAGTATTTTTTAGTACAAACTGCTGAATGTAGTCACGCAAAAGCTGTGACTTTGT
AAAACTAATTATTTTCTTTAAGGAAAACACGTGCGCGTGTAAGTAGCCTTTGCACAAAGTGCCAGTGTATATATATATATGTACACGTTCTCGTGTTAAG
AATTGTAAACTGGTTCTGTTGTGGGATTGGTGCAAATGAAGCATTTAATGGTTTTATATAAACAAAAAAAAAAACTTTTTAAAAAAATATTCTTAATATT
GCTGCTCAGACCATTTTTTGGTAAAGACACATTGTGTATGTATAAATAGATGTATTTTTGATATTGTTTGTCAGGGGTCCTGCTACTGACGCTGTGATTT
GTAATGTGACAATGTGACTGTGCTGTATAGTACTTAATGTGACAGTTGCTTTGGATGCACTGTTTGGGCATTCGTTCCATTGTTAAACAGTGTTCAGAAC
GGAGTCGAACGGAGCCATATTGACTTTTATGCAAAAGAGATTGTTTACATCTGTATCTGGAGGATATCCTTTTCCCAAAGTTGCCGAGACCAATTTGAGC
ACCTCCTTCTGCAACAGCCTTAACCTCTCTCATACCAACTGGATTTCTGTCTTCAAGACTGCACATTCCTTGAACTGTTAGTTGAACTGTTCCCTGTATG
CTTTAACAACAATACTGCCACAAGGGTAAGCATTGCTGGTATGGACAATATAATGGAGAGTTCAATGTATTAAAAATATAATTTTTATGTGTGCCCACAA
ATAGTCTGTATAAAAACCCTCACTGCTTTCTAACAGTTTTGCCCTATAGTTCACTTTCCCACTAGTAATGATGCTGGCATTTGTAGTATTGACTTTATAC
AACCAATATATATATAGAAGCTGCAACTTGTGTATTGTATGAAACTTTATGAAATGTATTTGGGCAAAGGCAGCTATTGGAAAAGGCTGGTGTCATTCTG
CTTTTCTCCCTCCTCCTCTTTTTTTTTTTTCTAAAGCTGCTGACTGTCTCGACAATGTTAACTTTCCTCCACTTTTTGGTGGCAAGCATTTCTGGAATAC
TGCAGTGCTGTATTCTGTTTTTAATTTTATTTTAATATACTCTAAGGAGTTAATTTGGATCACAATGTGATTTTTGTGCGAAAATAGTCTATTTATACAT
AGAGAATGTGTCAGCAGCACTTTGGTGTTAACCACATATAGAATGGGTTAACTACTTGTAACTGTTCTCCAAAGAATAATTGGCCAAAAAAAAAAAAAAA
A
5' EST: CF784960
[+]taattaatccccccccccccccccccggtgagttgcgatttggaggcgaagggtggaagtaggaggaaagattcgcaagtctgagagttttgcggtttat
ctgaattttgcttcgtttcttcactctccgctacgttcagtggggagaggagacatttctcgaaaagaagtctcgtttagaggacttcttccattgtaat
ccccggttgccttgtgcccagggggattcgatgtttgttttggatcttgataatcacatgcttaagaaaactagcagattaaagaaggcaacacaaagag
gggagcgagccttgttagttcggagacctggaaaggcagagaaggggtgtgaggaatcagtgggaaggtgacataaaagttggagatgggtggaggcgag
agatttaacattcctggtcagcatagaaataaccttggcaaacagattaaccgtcagaagttgttagagcgcaacaatcaaaagatgaacacgtctctca
gtaaggacagggtccgtggctgtgggacatctcttgcatggcaggccatgcagaatggagttaacaacaataacactcgttcctcgaatcaaaactggag
tgctggggtttccagcctccaagaacttctttcccgatcaagacaaccagaattacgctggagccaaatttagtgaaccaccatcaccgagtgtactttc
cccagccaccaaggccactgggtggctcttttccggcagtccagctgaaaaaagagctgatgtccttttcagctgaaaaacctttgctaaaggtcccggg
ccttaattttggacctttccaaaaccatc
3' EST: CF784959
[+]tttttttttttttttggccaattattctttggagaacagttacaagtagttaacccattctatatgtggttaacaccaaagtgctgctgacacattctct
atgtataaatagactattttcgcacaaaaatcacattgtgatccaaattaactccttagagtatattaaaataaaattaaaaacagaatacagcactgca
gtattccagaaatgcttgccaccaaaaagtggaggaaagttaacattgtcgagacagtcagcagctttagaaaaaaaaaaaagaggaggagggagaaaag
cagaatgacaccagccttttccaatagctgcctttgcccaaatacatttcataaagtttcatacaatacacaagttgcagcttctatatatatattggtt
gtataaagtcaatactacaaatgccagcatcattactagtgggaaagtgaactatagggcaaaactgttagaaagcagtgagggtttttatacagactat
ttgtgggcacacataaaaattatatttttaatacattgaactctccattatattgtccataccagcaatgcttacccttgtggcagtattgttgttaaag
catacagggaacagttcaactaacagttcaaggaatgtgcagtcttgaagacagaaatccagttggtatganagaggttaaggctgttgcaaaaggangt
gctcaaattggtctcggcaactttgggaaaaagatatcctccagatacagatgtaaacaatctcttttgcataaaagtcaatatgggctccgttcgactc
cggtctgaacactggtttaccaatggaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: