XB-CLONE-4351313
Clone Name: IMAGE:7026960
|
Gene:
cirbp
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CF784971 [+]
taattaatccccccccccccccccggcacttcgcattcggcttccaacttgtgttgtatcgtccgaagatcatagaacgtctcaccatgtcttgtgatga
aggaaaactctttgtcggtggtctgaattttgaaaccacagaggaaagcttggagcaagtgtttagcaaatatggacaggttgctgaagtggttgtggtg
aaggatcgggaaagcaagagatccagaggctttggctttgtcacatttgagaatcctgaagatgccaaggatgctatgatggcaatgaatgggaagtctg
tagatggccgtcaaatccgtgttgatcaggctggcaagtcctccaatgataggagaggtggttacagaggtggctcttctggaggcagagggttcttccg
tggaggcagaggccgaggtggtggtggagacagaggatatggtggaagcagccgctttgaaaacagaagtggaggttaccaaagcagtggatccagggat
tactatggcaggagtcatggaagctatggtgatcgttccggaggatcctacagggatagttatgacagctatactacacaagagtaaaatccattcctga
ctccagatcgtccttcccatggctgtatttataaagatttttgggagcttcgccgcaatcgttttggggtagtatatccaactttgtggttcaccttttt
ttttttttaataagaaccttaggtttagcccttgaacaacctttctctctaaaaaagttgggagacaaagcttgtttgtttttccaaaagggctttttta
tatcttttgtggaacacccccttatataccttcattttctcctttaaaagggactaaacggtggggtttttt
3' EST: CF784970 [+]tttttttttttttttcttttttttttttttttttttttttttttttttttttcttttcacattggtttttttaataggcttcgtgaagccaatcaaaagt
aaaatacaattccttagccacttcagtacaatagttgcatgtttagtaaaaaaaggacaatataaacaaacattttaagaacttcttctgatattgttac
gggaaatgcgaacactattcactttgtgtatcaaggaggggtagccactatatacagcgcacccaagctctgccttgccttgttacactaaaggaatgca
atttaagtggactcataaaattaacttttcatgtaaccaaaaaatacagcagcttaaagttctttaaacaatgctacaggaaaacaggcctagcagtctg
ctactaatacaggggcttctctgcccatcaaccaacagcacccatccgcttaggaagttacttcctagcactacttaaccagaagggggagcagccccac
ctgatcagcttgaatggaacagcatagtaggataggaatatccttgcaaaacacagtaactttaagaatgagattaggtgtccaaagataaagcctttga
aacacagctgtcccacttattaaagaagctgtcaggctaactaggtctattaaaaaaaaaagggaacacaagtaggatattctacacaaacgatttcggc
gaaactccaaaaaatctttataaaatacagcccattggaaaggacaaatcttggagtcagggaaggggatttttactcctgggggaaaaaaacactggcc
a
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
Suppliers: