XB-CLONE-4351301
Clone Name: IMAGE:7026956
|
Gene:
rpl4
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CF784979
[+]
taattaatcccccccccccccttttctcttcgtggccgctgtggagaagcagcgaggagatggcggtcagcatggcctgcgcccgtccgctaatatcagt
atactccgagaagggggaaacatctggcaaaaatgtcaccatgcctgcagtgttcaaggcacctattcggcctgatatagtcaactttgtccacacaaac
ctacgcaagaataaccgtcagccatacgcagtgagcgagcttgctggtcaccaaacaagtgctgaatcatggggaactggtcgagctgttgcccgtattc
cccgtgtgcgtggaggaggaactcaccgttccggtcagggtgccttcggaaacatgtgtcgtggagggcgtatgtttgccccaactaagacctggagacg
ctggcaccgtagagtcaacacaacccaaaagcgctatgcagtctgctctgcactggccgcctcagcccttcctgctcttgttatgtctaaaggtcaccgt
attgaggagatccctgaggttccccttgttgttgaagataaagttgagagctataagaaaacaaaggaagctgtccttctgctgaaaaagctcaaagcct
ggaatgacataaagaaggtttatgcgtctcagcgtatgcgtgctggaaagggtaagatgaggaacagacgtcgcattcagcgcagaggtccctgtgttgt
ttacaatgaaaataacggcattgtaaaagccttcagaaacatcccaggaatcaccctccttgaatgtaagccaggttgaaccttttttaaggctttgctc
ctggtgggtcatgtttggaacgga
3' EST: CF784978
[+]ttttttttttttttttttttttaaagtttgaaacaatttattgagtaaatccaaagtgctgaacaggaggaaacactccaggattactttgactctgctg
ctttagcttccgctctggcttgcctcttctttgctttggccaacttgatcattttagtttttgcatcaagtggcttctttgctttaggcttgccatcatc
tggcttcttttctttagctttaatattctcaagctgctgcaggatagcatgacgtcttgctgtctttgcatatgggttcagcctcatcatgattctcagg
ttctacagtgggttcttcttgagctcccttcgcttcacctttttgtttggagcccgcaaagccctctggatctcctggcttttcaggattctggtcaaat
ctgtgtttgtcatcttgtgcattggaaggttgtaatctgccttcagcttggctgatttgcgccatgtaccatagaggtcatccaatttgcggaaggcgct
ttctgtccaaatgcagaaccgtccaacatgaccaccaggagcaagccttaaaaggttcaacttgcttacattcaagagggtgatttcctgggatgtttct
gaaggcttttacaatgccgttattttcattgtaaacaacacagggacctctgcgctgaatgcgacgtctgttcctcatcttaccctttccagcacgcata
cgctgagacgcataaaccttcctttatgtcattccaggctttgagctttttcagcagaaggacagccttcctttgttttcttatagctctcaactttatc
ttcaacaacaaggggaaacctcagg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: