XB-CLONE-4351298
Clone Name: IMAGE:7026955
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Gene:
hbd
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077014
[+]
GAGAAGCCTCTTACTTCTCATATTAGAAGTCTTAGCAGTTACTTCCACCTACCGCCACCATGGTCCACCTATCAGCTGATGAGAAATCCGCCATTAATGC
TGTATGGCAGAAGGTCGATATTGAAAAAGATGGCCATGAAGCCCTCACCAGGCTGCTGGTTGTGTATCCCTGGACCCAAAGGTATTTCAGCAGCTTTGGA
AACCTCTCCAATGTGACTGCCATCTCTGGAAATGCCAAGGTTCATGGCCATGGCAAGAAGGTTTTGTCTGCTGTTGATGAAGCCATCCACCATATAGATG
ATATCAAGCACTTCCTTTCCGCTCTCAGCAAAAAACACGCTCAGGAACTTCATGTGGACCCTGAAAACTTCAAGCGCCTTGCTGATGTGTTGGCTATTGT
CGTGGCTGGTAAACTGGGAACTGCCTTCACTCCTCAAGTCCAAGCTGCCTGGGAGAAGTTCAGTGCTGGACTGGTGGCTGCTCTTAGCCATGGCTACTTC
TAAAGTATTTAGTTCACTGCAGAGTCCACCAACTGCTCATCTGTGACAAAGAGATATCTCACAATAAGTGCATTTCTTATTGTCAATAAAGATTTCTTGT
CAAACTGCGAAAAAAAAAAAAAAAA
5' EST: CF784981
[+]taattaatcccccccccccccgagaagcctcttacttctcatattagaagtcttagcagttacttccacctaccgccaccatggtccacctatcagctga
tgagaaatccgccattaatgctgtatggcagaaggtcgatattgaaaaagatggccatgaagccctcaccaggctgctggttgtgtatccctggacccaa
aggtatttcagcagctttggaaacctctccaatgtgactgccatctctggaaatgccaaggttcatggccatggcaagaaggttttgtctgctgttgatg
aagccatccaccatatagatgatatcaagcacttcctttccgctctcagcaaaaaacacgctcaggaacttcatgtggaccctgaaaacttcaagcgcct
tgctgatgtgttggctattgtcgtggctggtaaactgggaactgccttcactcctcaagtccaagctgcctgggagaagttcagtgctggactggtggct
gctcttagccatggctacttctaaagtatttagttcactgcagagtccaccaactgctcatctgtgacaaagagatatctcacaataagtgcatttctta
ttgtcaataaagatttcttgtcaaactgcgaaaaaaaaaaaaaaaaag
3' EST: CF784980
[+]tttttttttttttttcgcagtttgacaagaaatctttattgacaataagaaatgcacttattgtgagatatctctttgtcacagatgagcagttggtgga
ctctgcagtgaactaaatactttagaagtagccatggctaagagcagccaccagtccagcactgaacttctcccaggcagcttggacttgaggagtgaag
gcagttcccagtttaccagccacgacaatagccaacacatcagcaaggcgcttgaagttttcagggtccacatgaagttcctgagcgtgttttttgctga
gagcggaaaggaagtgcttgatatcatctatatggtggatggcttcatcaacagcagacaaaaccttcttgccatggccatgaaccttggcatttccaga
gatggcagtcacattggagaggtttccaaagctgctgaaatacctttgggtccagggatacacaaccagcagcctggtgagggcttcatggccatctttt
tcaatatcgaccttctgccatacagcattaatggcggatttctcatcagctgataggtggaccatggtggcggntaggtggaagtaactgctaagacttc
taatatgagaagtaaagctctcggggggggggggggggggggggattaatttaactcgna
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: