XB-CLONE-4351295
Clone Name: IMAGE:7026954
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Gene:
myl11
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784983 [+]
taattaatcccccccccccccatgtcactctctccttctgatcaggacaagcaccaagctcaaccgaggacatggcaccagcaaagaaggccaagagaaa
ggcagcagaaggtggatcttccaatgtgttgtccatgtttgaccaaacccagatccaggagttcaaggaggctttcacagtgattgatcagaacagagac
ggcatcattgacaaggaagacctgagggacacattcgcagctatgggccgcctgaacgtgaagaatgaagagttggaagagatggtcaaagaggcatctg
gccccatcaacttcaccgtcttcctgaccatgttcggcgagaagctcaagggtgctgatcccgaagacgttatcactggggcattcaaggtgcttgatcc
tgaggccaaaggatccatcaagaagcagttccttgaggaactccttaccacacagtgtgacagattcaccccagaggagatcaagaacatgtggacagcg
ttcccccctgatgttgctggcaacgtagactacaagaacatctgctatgtcatcacccacggagaggacaaagaccaggaataaacaggcccagtcttgc
cctcctgatggagggggtgcgccatcacgcccaacaccaaccttgctggtatattggcgccttattttataaacctcttcccatcaggagacacaaactg
taaatttatttccttcctaggggtgggtcctgaaaagggggacccccctccccgggtggtttcctggttccccctttttcccgcgcggttccccttttcc
accctccctcctttttaaaccccctccccttccacaattttttcgcgatc
3' EST: CF784982 [+]tttttttttttttttgttttgaataaaaagttgactttattgttcttcccttgagggctccagagttccagtttaagacatcaaagagcgaaatgtgagg
gagggtaaaagaggagggaaagggacagcgagaaaggggacaagaacaacagagaggggtcccctttcagaccacccctagaagaaataaattacagttg
tgtctctgatgggatgagtttataaaataagacgcaatatacagcaaggttggtgctgggcgtgatggcgcaccccctccatcaggagggcaagactggg
cctgtttattcctggtctttgtcctctccgtgggtgatgacatagcagatgttcttgtagtctacgttgccagcaacatcaggggggaacgctgtccaca
tgttcttgatctcctctggggtgaatctgtcacactgtgtggtaaggagttcctcaaggaactgcttcttgatggatcctttggcctcaggatcaagcac
cttgaatgccccagtgataacgtcttcgggatcagcacccttgagcttctcgccgaacatggtcaggaagacggtgaagttgatggggccagatgcctct
ttgaccatctcttccaactcttcattcttcacgttcaggcggcccatagctgcgaatgtgtccctcaggtcttccttgtcaatgatgccgtctctgttct
gatcaatcactgtgaaaacctccttgaactcctggatctgggtttgggtcaacatggacaacacattgggaagatccacctttcg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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