XB-CLONE-4351267
Clone Name: IMAGE:7026943
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Gene:
XB5846730
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785000
[+]
taattaatcccccccccccccgagctcatgttttgaggttccaagttctgttggagtgtgagctgagactttgaatctcttgcctcagagataactcgag
atgggtggcaggaaaaagaagacaagataagaagagccatcaagaaagttgtaaaatgggacatttgtggcagacgtccagttccaaaagaattaccaca
agtggattgtgtaatcagcctctggattctaaatataataagtaaaaccaaggctgaattcaagatcaatctaaaaagtttggtgtcccagttaaaaact
gggggttatttaatgttttttgcagtcataaatatgacttattatatatttgggtgggcataagttctttgttctggcacctgatgaaaatttttttaag
ggaggctgcccgaaaaagccgggcctagtcctttcaaaagttttgaaattgcagcctgaaactcaaaagtccaccacctttggggggggaataagaaaaa
aaggggggcttttttctgttttacccgccccccgggggaaaagggggagggcccgggaataccccccaaattttataaaaaaacccgggcaaaaaaaaaa
aaaggggttttcccgggggaaagaaaaaatcccgttttttttttctcagggggccaaccccaaaaaaaaatctcccccctatatagggcccccaagaaac
cccccccaaaaaattttctctttattaaggggggggggcgcaaaaaaaaaaaatcattttcggggaggagggaaaaaaat
3' EST: CF784999
[+]tttttttttttttttgcagatattgcaagaatttatttttacttcagatatgttatttgccaacattaaatgaatttgtgggttctgtgctatatggagt
atatcagcttgcctgacaataactgatctacttccaggaagccttatttcttgcagatttataaattaggatatcagtcatccttttccttgcgcgctaa
cagaaaagccactttatcataatccaccaaagtgctggactttgagctcagctgcaattcaaacttctgaatgactagccctgcttctctggcagcctcc
cttaaaaaattttcatcagttgccagaacaaagaacttatgcccaccaaatatataataagtcatatttatgactgcaaaaaacattaaataacccccag
tttttaactgggacaccaaactttttagattgatcttgaattcagccttggttttacttattatatttagaatccagaggctgattacacaatccacttg
tggtaattcttttggaactggacgtctgccacaaatgtcccattttacaactttcttgatggctcttcttatcttgtcttctttttcctgccacccatct
cgagttatctctgaggcaagagattcaaagtctcagctcacactccaacagaacttggaacctcaaacatgagctcggggggggggggggggggggatta
aattaacctcgagtggcaaaaagaaaccaagtagcttgggtattcnggccccggaatagaacccgaaggtggcagttggggaccctgggaagtgggacac
cntgntgggagagaaaaggccaaaagntggaatgggtttattccctataagtgtc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: