XB-CLONE-4351264
Clone Name: IMAGE:7026942
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Gene:
myh4
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785002
[+]
taattaatcccccccccccccgcttctcagtctctgggggacacaacctttttttttgtcatcatcatctgtattaaaaggaagcagcaatcatgggtga
tggagaaatgtccatcttcggcgaggccgcacaatttctcagaaaaagtgagaaagagcgtttggaggctcagagcaagccttttgatgctaaaaacact
gtatttgtagatgatgttaaggaactctatgtcaaaggtatggttacagccagggaagatggcaaaataactgtgaagactgatgatggaagaacagtaa
ccgtcaaagagaaccaaatttacccccagaaccctcccaagtttgataaaattgaagacatggctatgatgacccatttgaatgaggcttctgtcctgta
taacttgaaagagcgttatgctgcatggatgatctatacctactctggccttttctgcgccactgttaacccctacaagtggctgccagtgtacaaccct
gaagttgttgccggttacagagggaaaaagcgtatggaagctccccctcacattttctccctttctgataatgcctatcaggccatgttaacagatcgtg
aaaaccagtctgtccttattactggtgaatctggtgcaggaaagactgtgaacaccaagcgtgtcttccagtattttgcaccattgcagcccttggagac
aagaagaaagaatccctccaacagctttgcaggggaaaatctggaaaaatccagattatttcagggccaacccctcttgctgtgaagacctttgggtaaa
ttgcccaagaact
3' EST: CF785001
[+]ctttttcttaccacctttgccaccagcatcaccgtcagttgcagcatagctggagtagagcaaggacagcagtttgacagaagacttctggtagagctgg
acaactgactcgttcagtgggtccttgttcttttcaagccatccactgatgttgtaatccacagtgccagcatagtgcacaagcgagaagtgagcttcag
cctttcctttgccaggcttgggcttctcaaagttcttgcatttgcccagatgttgctcatatagcttgttcttgaaggaagtatcagtggctttggggaa
catgcactcctcttcaaggatggagaagatacccaatggcttctcaataagctcaatgcaggcagccaaatccataccgaagtcaataaactcccaatca
attccttccttcttgtattcctcctgctccaggacgaacatgtggtgattgaaaaactgttgcagtttttcattggtaaagttgatgcagagctgctcca
agctgttaaaatcaaagatttcaaatccagcaatgtccagcacaccaatgaagaactgtcttggttgcttagtatccagctgttggttaatacgagtgac
catccacaagaacagcttttcaaaaatagacttgcacagggcaccaacagagttatagacctgtggtacagtctgtcctttggtaaacaattcattgccg
accttgactcttgggtagcacaaacctttgagcaaatccgcagagatgaggccccaacagataagcaattttgtcagcca
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: