XB-CLONE-4351250
Clone Name: IMAGE:7026936
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Gene:
tpm1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785010
[+]
taattaatccccccccccccccccccgatccttgctctctgctttgtcctagctgacttgtcggagaatatctaccaataaacagccatcatgacttcct
ataggtccagctctgcttcatactattcaagctcatccggcaaaggtggctttggaggcagcagctatggaggcagcagcttggcaggcagcggctttgg
aggaggctttggcagcggtgcaggctctggcttctcatcctctgggggtaactttgccatggcggcggcagccagctccagcttaagcaacttcggtgga
aatgaaaagcacgccatgcagaacctcaatgaccgccttgcttcctacctggagaaggtcagagccctggaagcctccaatgccgacctggaagggaaaa
tccgcacttggcactaaaatcaaactggcaccggccttggagctgggtctaaagactacagcaaatattttgagatcatcagtgaagctgaagggcaaaa
tccacggtgcaactgttgataacgcttacagtgacccgtgccaattcgacaacccccagaatgggctggctgaacaacttcagaacttttaatttgaaaa
atgagcctgggctccttcccccaaaactgtggtaggcttaatatcgcctgggccttgagaaaaagcccctgggaaggaaccggtaaccctggtttaaaaa
gtagaccttggaagatgcctctattggatgagcttttacatgaaaagatctggggtctaaccctgaaagaagaaaccattgtgggagagaaaatgtaatg
ctttggccaaaatagcccaan
3' EST: CF785009
[+]ccctgcatcttctgtcaagttctctctgtcaccctaaccccccacacaagcagcagccatggacgccatcaagaagaagatgcagatgcttaaactggac
aaggaaaatgccttggacagagcagaacaggctgaagcagacaagaagggagcagaggagaagagcaaacagctggaggatgaacttgtagcattgcaga
agaaactaaaaggtactgaggatgagctggacaaatactctgaagccctgaaagatgcccaggagaagctggaacttgcagagaagaaagccactgatgc
tgaaggagatgtagcctcattgaacaggcgcatccagctggtagaggaggagttggatcgtgctcaggagcgtctggccacagctcttcagaaactggag
gaggctgagaaggctgcagatgagagtgagagaggtatgaaggtcattgaaaacagagccctgaaggatgaagagaaaatggaactgcaagaaatccagc
tgaaagaggccaagcacattgctgaggaggctgaccgcaaatatgaagaggttgctcgtaagctggtgatcattgagggtgacctggaacgtgcagagga
acgtgctgaactttcaaaaagcaaatgtgccgagcttgaggaggaattgaaaactgttaccaacaaacctgaaatctctggagggccaggcagaaaagta
ctccccagaagggaggacaaantatgaaagagggaaatttaagggtttcttaccagaataaacctggaaaggagggcttgaaaaccccgtggccccaaga
ttttgccanaaaaaggaaccgttaacccccaagttttgggaaaac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: