XB-CLONE-4351244
Clone Name: IMAGE:7026934
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Gene:
cirbp
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785014 [+]
taattaatcccccccccccccggcacttcgcattcggcttccaacttgtgttgtatcgtccgaagatcatagaacgtctcaccatgtcttgtgatgaagg
aaaactctttgtcggtggtctgaattttgaaaccacagaggaaagcttggagcaagtgtttagcaaatatggacaggttgctgaagtggttgtggtgaag
gatcgggaaagcaagagatccagaggctttggctttgtcacatttgagaatcctgaagatgccaaggatgctatgatggcaatgaatgggaagtctgtag
atggccgtcaaatccgtgttgatcaggctggcaagtcctccaatgataggagaggtggttacagaggtggctcttctggaggcagagggttcttccgtgg
aggcagaggccgaggtggtggtggagacagaggatatggtggaagcagccgctttgaaaacagaagtggaggttaccaaagcagtggatccagggattac
tatggcaggagtcatggaagctatggtgatcgttccggaggatcctacagggatagttatgacagctatactacacaagagtaaaatccattcctgactc
aagatcgtccttccaatggctgtatttataaagatttttggagcttcgccgaatcgtttgtgtaggatatctacttgtgttcacttttttttttaataga
cctagttagcctgacagcttcctctaataaagtgggacaactggggtttccaagggct
3' EST: CF785013 [+]tttttttttttttttcaattggtttttttaataggcttcgtgaagccaatcaaaagtagaatacaattccttagccacttcagtacaatagttgcatgtt
tagtaaaaaaaggacaatataaacaaacattttaagaacttcttctgatattgttactggagatgcgaacactattcactttgtgtatcaaggaggtgta
gccactatatacagcgcacccaagctctgccttgccttgttacactaaaggaatgcaatttaagtggactcataaaattaacttttcatgtaaccaaaaa
atacagcagcttaaagttctttaaacaatgctacaggaagacaggcctagcagtctgctactaatacaggtgcttctctgcccatcaaccaacagcaccc
atccgcttaggaagttacttcctagcactacttaaccagaagggggagcagccccacctgatcagcttgaatggaacagcatagtaggataggaatatcc
ttgcagaacacagtaactttaagaatgagattaggtgtccaaagataaagcctttgaaacacagctgtcccacttattagagaagctgtcaggctaacta
ggtctattaaaaaaaaaaagtgaacacaagtagatatactacacaaacgattcggcgaagctccaaaaatctttataatacagccattggaaggacgatc
ttgagtcaggaatggattttactcctgtgtagtatagctgtcaaaactattccctgtaaggatcttcgggaaacaatcaccatagcttccatgaactccc
tgccataataaacccctgaaatccactgcctttga
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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