XB-CLONE-4351241
Clone Name: IMAGE:7026933
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Gene:
rpl9
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785016
[+]
taattaatccccccccccccccttttcctctgtcagctgcgagaatgaagaccattctcagcaaccagattgttgacatcccagagaatgttgacatctc
tctgaagggccgtacagttactgtaaaggggcccagaggtgtcctgcggaaaaactttaaccacatcaatgttgagctgtgtcttctgggcaagaagaag
aggaggctccgggttgacaaatggtggggtaacagaaaagaactggccacagtgcgcaccatctgcagtcatgtgcaaaacatggtgaaaggagtcacac
tgggctttcgttacaaaatgagatctgtgtatgctcactttcccatcaacgttgtcattcaggagaatggatctcttgtggaaataagaaacttcttggg
tgaaaagtatatccgcagggttcgcatgagatcaggtgttgcatgcgccttatcccaagcccagaaagatgagctgattcttgaaggcaatgacattgaa
cttgtatcaaactctgctgccttgatccagcaagccaccacagtaaagaataacgatatcagaaaattcttggatggtatctacgtatccgaaaaaggga
cagtacagcaggttgaagaataaaagcctgcaaaagtctggtttcctgtgctggtccaaaaaataaaatcccctttatctaaaaaaagaaaaaaaaaaaa
aaaaaagggtcccccctaatttcttagaagcgggccggccaccccgggggtgggaaacctttggcccgttgggccccaaacttttggtttaattttggca
gcctttttaataatgggggtttaaccaaaacacaaacgcgccaattatctcactccctccacaattct
3' EST: CF785015
[+]tttttttttttttttcttttttagataaagggactttatttttggaacagcacaggaaccagacttttgcaggcttttattcttcaacctgctgtactgt
acccttttcggatacgtagataccatccaagaattttctgatatccttattctttactgtggtggcttgctggatcaaggcagcagagtttgatacaagt
tcaatgtcattgccttcaagaatcagctcatctttctgggcttgggataaggcgcatgcaacacctgatctcatgcgaaccctgcggatatacttttcac
ccaagaagtttcttatttccacaagagatccattctcctgaatgacaacgttgatgggaaagtgagcatacacagatctcattttgtaacgaaagcccag
tgtgactcctttcaccatgttttgcacatgactgcagatggtgcgcactgtggccagttcttttctgttaccccaccatttgtcaacccggagcctcctc
ttcttcttgcccagaagacacagctcaacattgatgtggttaaagtttttccgcaggacacctctgggcccctttacagtaactgtacggcccttcagag
agatgtcaacattctctgggatgtcaacaatctggttgctgagaatggtcttcattctcgcagctgacagaggaaaggggn
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: