XB-CLONE-4351221
Clone Name: IMAGE:7026924
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Gene:
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785028 [+]
taattaatcccccccccccccccgttgtgctgtgtggttgctacagaaaccctactatagtttatataaataccccgctgtgtagccccgggggcagcca
ttcctgcactggtacagctggggtgtttgctacagaaaccctactatagtttatataaataccccgctgtgtagccccgggggcagccattcctgcactg
gtacagctggggtgtttgctacagaaaccctactatagtttatataaataccccgctgtgtagccccgggggcagccattcctgcactggtacagctggg
gtgtttgctacagaaaccctactatagtttatataaataccctgctgtgtagccccgggggcagccattcctgcactggtacagctggggtgtttgctac
agaaaccctactatagtttatataaataccctgctgtgtagccccgggggcagccattcctgcactggtacagctggggtgtttgctacagaaaccctac
tatagtttatataaataccccgctgtgtagccccgggggcagccattcctgcactggtacagctggggtgtttgctacagaaaccctactatagtttata
taaataccccgctgtgtagccccgggggcaaccatttctgcactggtacagctggggtggttgctacagaaaccctactatagtttaaataaataccccc
gctgtgtagcccccgggggcagcccattcctaatagcagggtttcctttagtaatcacaaacaacgttttcc
3' EST: CF785027 [+]tttttttttttttttcaatgttttttattcatactgcacaaaatacagcaataataaaaaatacaacatttctgccatttttaatatactacagtgtgca
tgattttttcattcattttaaatctagccttctatctttcctattattctaaaggcatcccaggactgacattgcaatgtacagtactcattggtgtgtg
catatacacctgccctacagcatggggtcttttgcggagaaatcaagtctaaagtacaatgatccaaaatgatgatgtatttgtaaaacaaatatatatc
tttgagttcataaacttgttgaaattttaaataactgtgttatatctgcacagaatgattttgtcctcagagaggctcactacactctttgttaaaggaa
cagtaacaccaaaaatacaaatgttttaaaatgattaaagtataatgtacagttgccctaccctggaaaacgttgttgtgattactaaagaaaccctgct
ataggaatggctgcccccggggctacacagcggggtatttatataaactatagtagggtttctgtagcaaccaccccagctgtaccagtgcaagaatggc
tgcccccggggctacacagcgggggtatttatataaactatagtagggtttctgtagcaaacaccccagctgtaccagtgcaggaatggctgcccccggg
ggctacacaggcgggggtatttatataaaactattagtaaggtttttctgtaaccaaacaaccccagcctggtccnagttgcaaggaaatgggctgcccc
ccggggggcttacaccagcaaggggtattttatata
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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