XB-CLONE-4351213
Clone Name: IMAGE:7026921
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Gene:
bpnt2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC080464
[+]
ACTTCCTAATTCGCTATGGCCCCTATGGGTATCCGTCTCTCCCCGCTGGGAATCGGAGTGTTCTGCTTGCTGGGGCTGGGAGTGCTGTACCATGTATACT
CGGGCTTCCTCACCGGCAAGTTCTCAGCCTTCCTCCTGGGGGACAGAGCGGAGGATCCCGGGCCCGGGGAGGACACAGTGGATCTGAGGGAGCTGCTGGC
TGTGTCGGTGCGGGCTGCGGAGCTTGGGGGGCTGGAGGTGAAGAAGGTCCGGGAAAGTAACTCGCTCAATGAGAAGGCGAAGGGCAAAACCATGGAGGGG
GCGGACGACAAGATGACCAGTGGGGATGTGCTGTCCAATAAGAAAATGTATCACCTCATCAAAAATGCCTTCCCGGCCCTGAAGGTGAACACTGAGGAAA
AAGTTGAAGCAGATGATGAAGATGCGGTCTCTTGGGATCGTAATATTCCTGATGATATAAAGGAGCAAATAAAGACAAAACATGTTGCATCTGAAAGCAT
TACAATGTGGATAGACCCACTAGATGCAACACAGGAGTATACAGAAAATCTGGTAAACTATGTGACTACCATGGTATGTGTCGCTGTAAATGGCAAACCT
GTTATTGGCGTAATCCATAAACCTTTTACAGGTTTTACTGCATGGGCCATGTTGGATGGAGGTTCAAGTATAAAGAAACGAAATTCATACAATGAGAAAA
CTCCCACTTTTATTGTGTCACGTTCTCATTCGGGAGAAGTGAAAGAGGTCACCCGGCAAACATTTGGTAACAAGACAGAAATCATATCAGCAGGAGGAGC
AGGTTACAAGGTTCTTTCACTGCTGGATGTGACTGATGATAAGCAAGAAACTGCTGATGTGTATATTCACGTCACCTACATTAAGAAGTGGGACATTTGT
GCTGGCAATGCAATCCTAAATGCACTTGGAGGTCAAATGACTACTCTTAAAGGAGAAGAAATTATGTACACAGGATCAGAACTGAATAAAGGGGGGCTGC
TAGCCAGCATTGGAATGGACCACGGTGTGCTTGTTGAAAAATTAGCTGAAAAATTACAAGTTAATGCTAAGAAACCAGCCAAGTAAAGGATAACTGAAAT
AAATTCCTACATTGCGCGTTATGACTTTGGCTTGATGTGTTAACTAGAATACGCGAGGCCAGCATGTGAAAGACAGGGAATTGCAGTGCTGCTCTAATAT
GGCAGTGAAGGACTATGGGCTCCAGGAGTTTTCCTTCTGTATGCTTCAGTGCAAGGTGCAATATACTGCAAGTACCCCAACTGTCTTATTAGGACTTCAA
AGACATTGTTGGCTTTGGATATGGACGGTGCATTTCATGAACATGTAAATCTGTAAAGTCTGATTTCAAGCAGAATATGAATAGAAGTTTAATTTTGCCT
AATTAAAGTTGCGTCAATAGACGGCCTTTATTCTTTTTTTTTTTTAATTCCAATTTCTCATTAGCCTGATTAACAAAAACAATCATTGTGCCTTGTTGCT
GACTTAAAAGTATAGACATCTTGCGACTTAAACCTATCCTGATGAATACAATGTTTTATTCATTGTAGTGTTTTTGTCTAGAGCTTGAATAATGGGTTTT
TTTCTGTTCAAGTAGCAGCCACGTCTGTATTGTATAGAGAAATACTGCAAAGTCATTACTAATTAATAATGCATATTCTATCACATAGGAATTCATTGTT
TCACCAGGACTGTGTGTACATCTCATTGTAGGCGCACTGCTTCCCTACACTCCCTTAACTGTGTGTTTTCTCTTTGGGTTTTAACTGTAAATGGAACCTA
TTCAATTTTGGAGGCATTCTAGATGTTAATAAGATAATAAATCACTGTCATTTTGAAAAAAAAAAAAAAAA
5' EST: CF785033
[+]taattaatcccccccccccccacttcctaattcgctatggcccctatgggtatccgtctctccccgctgggaatcggagtgttctgcttgctggggctgg
gagtgctgtaccatgtatactcgggcttcctcaccggcaagttctcagccttcctcctgggggacagagcggaggatcccgggcccggggaggacacagt
ggatctgagggagctgctggctgtgtcggtgcgggctgcggagcttggggggctggaggtgaagaaggtccgggaaagtaactcgctcaatgagaaggcg
aagggcaaaaccatggagggggcggacgacaagatgaccagtggggatgtgctgtccaataagaaaatgtatcacctcatcaaaaatgccttcccggccc
tgaaggtgaacactgaggaaaaagttgaagcagatgatgaagatgcggtctcttgggatcgtaatattcctgatgatataaaggagcaaataaagacaaa
acatgttgcatctganagcattacaatgtggatagacccactagatgcaacacaggagtatacagaaaatctggtaaactatgtgactaccctggtatgt
gtcgctgtaaatggcaaacctgtttattggcgtaatcccataaacctttttacagggttttactgccatggggccatggttggcatggaaggttccaagt
tataaaagaaaacgaaaattccttaccaatggaagaaaaattcccccccttttttatttggtgtccacgtttctccctttccgggaagaaagtggaaaaa
aaggtccacccccgggccaaacaatttttggcgtaaaagaagaaaaaaaaa
3' EST: CF785032
[+]tttttttttttttttcaaaatgacagtgatttattatcttattaacatctagaatgcctccaaaattgaataggttccatttacagttaaaacccaaaga
gaaaacacacagttaagggagtgtagggaagcagtgcgcctacaatgagatgtacacacagtcctggtgaaacaatgaattcctatgtgatagaatatgc
attattaattagtaatgactttgcagtatttctctatacaatacagacgtggctgctacttgaacagaaaaaaacccattattcaagctctagacaaaaa
cactacaatgaataaaacattgtattcatcaggataggtttaagtcgcaagatgtctatacttttaagtcagcaacaaggcacaatgattgtttttgtta
atcaggctaatgagaaattggaattaaaaaaaaaaaagaataaaggccgtctattgacgcaactttaattaggcaaaattaaacttctattcatattctg
cttgaaatcagactttacagatttacatgttcatgaaatgcaccgtccatatccaaagccaacaatggtctttgaagtcctaataagacagttgggggta
cttgcaggtatattgcacncttgcacttgaagcatacagaaagaaaactcctggaagccccatantcccttcactggccatatttagagcagcactgcaa
attcccctgtcttttcacatgcttg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: