XB-CLONE-4351205
Clone Name: IMAGE:7026917
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Gene:
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785038
[+]
taattaatcccccccccccccgaaggagcctgagagaaacaagatgaaccccatggtgctgtgctctgtgtttgtggctgccatacttcagggcactgtg
tctaacactacaaagaagctcgatccaggcactacaactgtgatgccgaatttcatacctccactgttatacaggctgcagtgctactcttgtcagacac
gttcttgtcatgtttggaataaagtgacgtgttcctatggggatgtgtgtatggcaatctatcgtaactcagcgcagaatgaaaaccgtttggtatcctg
gagtggctgcaagaaagggctatatcgcaaatcaacacatggggccttgggaacaaaaagagtgctgtgctgcacaacgaaccactgctataactaacga
taaaatcctgaatttgcaaccacactgcctgtcctatactacagaaacactgtttaaaagttattccctgctaaatgcaccgcaaccatttttccacttc
ttcgccacacacgaagcccctgcataattgactattctattggactccccatctccgtttttttttcccccccctgtttggccccccacacctttttcgt
ctataacccctccttaacggtttatataaaaagcgtcaaattcctttctccaaaataaattttttaccaacccccgttatcttcttaacttttatattac
aacagaaccgccctatttccccacgtggaaacctttttgcccccccgtgctccctatcttacatttttttttcccaccctttttagtattggcttttaaa
aaccactaaaccccacccatattaccctctataccccccacacaatacaaattctcccactatttttatcccgcaccttccccatttttcttccgccctc
tttcactaaaatctgtatcccaccccacctttttcatctatttttccttgtcacacctgttn
3' EST: CF785037
[+]tttttttttttttttctgtagatttagctttattaacatgagcagcgtcagccgttatgttggctccaaacaggaaaaaaaaacagaagtggagtccata
gattatcattatgcagggcctcctagtggcaaaagtggtgaaatgcttgcctgcatttagcaagcaataacctttgaacagtgtttctctccatataaca
gtcagtttgttttccaattcaagattttctcattagttattgcagtagttccttgtgcagcaccgcactctttttgttcccaaggccccctgtgttgatt
tgcaatcttgccctttcttgcagccactccaggataccaaacggttttcattctgcgctgagttacgatagattgccatacacacatccccataggaaca
cgtcactttattccaaacatgacaagaacgtgtctgacaagagtagcactgcagcctgtataacagtggaggtatgaaattcggcatcacagttgtagtg
cctggatcgagcttctttgtagtgttagacacagtgccctgaagtatggcagccacaaacacagagcacagcaccatggggttcatcttgtttctctcag
gctccttcggggggggggggggggattaattaac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: