XB-CLONE-4351199
Clone Name: IMAGE:7026915
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Gene:
tmem70
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785042
[+]
taattaatcccccccccccccgagtgtgactgtgtgagtgagcctgaggagcccggggtacagacatgttccgcctcacaacttgctgccgccacagcct
tgcgctcagccgggccagactcagccttgcaggctcccggtcagctgcagtgtatgggctcctatccggtaaggagggacatggctctgcgcggaggagc
tgtgcgccttatacacagattattctacctaatgtgacatccgtccgatgttttggcacttcaccggtttctgggaattatgaagatggcagattaattt
acactgggaatctagcaaaatcagttcttgggataaagtttttctcgtattccaccagtatttttagtatttgcctgatgccctacattatgctgaaatc
tggaatcagtgttgacagtttggcattaaagatcgcctttatcagtgtcgtaggcttcttcacatttgtcactccagttactttgcacttgattacaaaa
ggctatgtggtccggttataccacaacgcagagacagatacctacacagcagtcacttacaatgcccctctttctgagaaaaggacagtttttcatcaaa
aggatgttgaaataccccgagtaagcaagatgttcacaacgttttacgctcaaaagaaatcaaggtgggtgaatcccaaggcttttttggaaaatcctta
tgaataataaaccttctaatggggctatgaataagccctttttaaaatttcagcccctggaaaaaaatttgagtcccgttctttcttggagggattaaac
taaaaacaaaaaaaaaaaaaagggattccc
3' EST: CF785041
[+]tttttttttttttttatttatcctcagaagaccgactcagttcttcaggactgaatgtaaaaggcttatcatagcccattagatgattatagtcataagg
atttccaaaaagcatcggattcaccaacattgatttcttttgagcgtaaaacgttgtgaacatcttgcttactccgggtatttcaacatccttttgatga
aaaactgtccttttctcagaaagaagggcattgtaagtgactgctgtgtaggtatctgtctctgcgttgtggtataaccggaccacatagccttttgtaa
tcaagtgcaaagtaactggagtgacaaatgtgaagaagcctacgacactgataaaggcgatctttaatgccaaactgtcaacactgattccagatttcag
cataatgtagggcatcaggcaaatactaaaaatactggtggaatacgagaaaaactttatcccaagaactgattttgctagattcccagtgtaaattaat
ctgccatcttcataattcccagaaaccggtgaagtgccaaaacatcggacggatgtcacattaggtagaataatctgtgtataaggcgcacagctcctcc
gcgcagagccatgtccctccttaccggataggagcccatacactgcagctgaccgggagcctgcaaggctgagtctggcccggctgaacgcaaggctgtg
gcggcagcaagttgtgaggcggaacatgtctgtaccccgggctcctcnagctcactccacagtccctcn
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: