XB-CLONE-4351196
Clone Name: IMAGE:7026914
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Gene:
rpl23
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785044
[+]
taattaattccccccccccccggcccttgcctgacttgctgctatatcccagcatcctttgcgtccgcctctttctctttccggtaaccaagatgtctaa
gataggacgtggaggttcgtctggtgcgaagtttcgcatctcccttggtctccccgtgggagcagtcattaactgtgccgacaacacaggtgcaaagaat
ttgtacataatctctgtgaaaggcatcaagggtcgcctgaacagactgccggctgctggtgttggagacatggtgatggacacagtaaagaaaggaatac
ctgatctgatgaaaaaggtgcatccggcaatggtaatacagcaacagaagtcctatcagaacaaataaggggtgttcttagtattttaaagacatatgcg
ggggtgatattaattaaaaaaagcggaaaaagaaagtgctcatctattatatggcacccgtggctccaaaaaatttatgcaaaaaactaatatgcacaca
atatttgcttaccctaacgtcaggtgtattcaatacaggttttgatagaaccaccccatttatccatgtttttaataaactatatacaagtataagtttg
ttgttaagcttaaaataatgttccttggtaattaagaacaggttttacctacttttatttttctttaatgatcgaccgagtgcgtcagatccacatcaag
aattggggcgattggtaaaaatcagttatatagtaatgcttatattagctctcccagatcttttttgcgttatttagttatattcctaagcctattttaa
taaaaaaggcgatttataaaataaatagctgtgtaacaagtagaagaaagaaacaagttttgtacctaatatgtttttgtctctattcanagagagagag
agtaggattttattgttataaaggacttgtagcatcaatattgtagaattgaaatagggggtgaatagaatgcttgaaaaaatctgtcatgtattacgtt
cagtattgtgaatgttctaaccccacatgcatctgatgatgatctgagcgataaaattgtatgtattctcagttttgtctcatacacacctttctctcgc
atatttttaatacaataatcatcattatgtgtataatagaatatgaattttctta
3' EST: CF785043
[+]tttttttttttttttgttccaacttttttttattttacaaagagagggtgtgttcatgcgatgctgcctgcgttggaagcaatcctgggccacagatctg
cacactctttcgccacggggcctgtgatagctgagcctttcatctcccccttgttgtttactatcacccccgcattgtcttcaaatacaagaacaccccg
tcttttctccggtacgacttccgctgccgtattaccactgccggatgcaccttcttcctcagctcaggttttcctttctttactgtggccatcaccatgt
ctcccacaccagcagccggcagtctgttcaggcgacccttgatgcctttcacggagattatgtacaaattctttgcacctgtgttgtcggcacagttaat
gactgctccgccggggagaccaagggagatgcgaagcttcccaccagacgaacctccacgtcctctcttaaacatcttggtttccggaaaagagaaagag
gcggaaggccaggatgcctgggattataataaaccagttcaggccaagggccctgaaaggggggggggaaaataaattaaccgccgaggtgctaaaaaga
aaaaaagtcccctttgttagtccggggcccccggaaaaagagcccaaaggggggccactttgggaccctggggaaagggggacactcttttgtggcaaag
agaaaagggcgcaagagcgggaaagatttaaattctataaaggttggaaaccccaaaaaaaatcccaacacgtggggaaagaacaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: