XB-CLONE-4351193
Clone Name: IMAGE:7026913
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Gene:
eif5a
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785046
[+]
aatctcccccccccccccacaacccnnggggttctttttttttgttggaaggtaaggaaggtactggtagacacccagcaacatggcagatgatatagat
ttcaccactggagatgctggggcttcctctactttccccatggagtgctctgctctgcgcaagaatgggtttgtggttctgaaaggacgtccctgtaaga
ttgttgaaatgtcaacttccaagactggcaaacacggtcatgccaaggtccacctggttggtattgatatctttactggtaagaagtgtgaagatatttg
tcctttctcccacaacatggatgttccaaatattaatagatgtgattaccaactgattggtattaatgaaaattacctggcccttatgagtgataatggt
gatgtcgtatgaggatccgtacatgccagagggtgcatttgggaaggggaacacgcttgtagaacacacgattgcaaagtagaagacaatctttggtcca
catcttctggataggttaaggacatgaaaaaattggtgtttaaaagtcacctactgagtgataatggttttataaatgaaaatagtgggaacctcacggt
gtaagttttcttagacatgtgagagtctttccaacctcggtctggaatggataaattctattttgagcatggtagagtgactcctaaacgtttagttggc
gtggagtagataggaggagttatataagaatgtggaccatcccaacatcttctaatagggtcaagtataccgtctgtttatcgatatgatgatgtagttg
aaaatatgtaacacgagagtttcagtatatgatanacaagagaaactaactaaaaatgatgaggggggattatatcaccactagcggcatataactattt
gtgtgcatcttttgattcatcagatccggagttattctgccaggaatatacatcgttctgaacaaaagtactcttgctaaggaccctgtaatagtaaaca
ctgtcgtaagatcaaaatgaacaagcctgtgtattcgg
3' EST: CF785045
[+]tttatttagtcattgccttgagggctacagcacattcttcattcattgcagtcaagactgtgaccaagatctcctctcctccatcatgtttcatcagtat
ttcccttcccaaatctccctctggcatcttcagatcctcacgaacatcaccactatcactcagaagggacaggtaattatcattaataccaatcagttgg
taatcacatcttttaatatttggaacatccatgttgtgggtagaaggacaaatatcttcatacttcttaccagtaaagatatcaataccaaccaggtgga
ccttggcatgaccgtgtttgccagtcttggaagttgacatttcaacaatcttacagggacgtcctttcagaaccacaaacccattcttgcgcagagcaga
gcactgcatggggaaagtagaggaagccccagcatctccactggtgaaatctatatcatctgccatgttgctgggtgtctaccagtaccttccttacctt
ccaacaaaaaaaaaggacccgggggggggggggggggggggggggn
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: