XB-CLONE-4351190
Clone Name: IMAGE:7026912
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Gene:
ston1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785048 [+]
taattaagccccccccccccgctcattgcaagctttacaagctcggagacggcgagcagctcatttaaaaccagtcgtccatatcacattactgagagca
acaatgtctaagggaccagcagttggaattgatcttggcaccacttactcatgtgtgggtgtcttccagcatggcaaagtggaaatcattgccaatgacc
aaggaaacaggaccacacctagttatgtggcttttacagatactgagagattgattggagatgcagctaaaaatcaagttgccatgaaccccacaaacac
agtttttgatgccaagagactcattggccgcagatttgaagatgcagttgttcagtctgacatgaagcactggcctttcaatgttatcagtgacagtgga
agaccaaaggtccaggtagaatataaagcagagaccaaatctttctatccagaggaaatatcatctatggtcttagttaagatgaaggaaatagctgaag
cataccttggcaagactgtaacaaatgctgtaattactgtcccagcatattttaatgactcccagcgacaggctacaaaggatgctggtactatatctgg
gctgaatgtactgcgaatcattaatgagccaactgctgctgccattgcttatggacttgataagaggttggagcagaaagaaacgtgctgatctttgatc
ttggggggggcacttttgatgtctccattccaaccattgaagatgggctctttgaagtaaagtctaccgcttgtggacactcattttgggggggtgaaaa
aatttc
3' EST: CF785047 [+]ccaaccaatacaatatcgtgtatttgtgacttgtcaagcttagcatcacgaagtgctttttccacagggtcaagggtacctctaaagagatcagcattaa
gttcttcaaaacgtgctctggtgattgaggtatagaagtcgattccttcatagagagaatcaatttcaatgctagcctgagtgctggaggagagcgtacg
ctttgcacgctcacaagcagtacggagacggcgaacagctctcttgttgtcaatgatgtccttcttgtgctttctcttgaattctgcaacaaagtgattc
accatacgattatcaaagtcttcaccacccaaatgagtgtcaccagcggtagactttacttcaaagatgccatcttcaatggttagaatggagacatcaa
aggtgccaccaccaagatcaaagatcagcacgtttctttctgctccaccttcttatcaagtccataagcaatggcagcagcagttggctcattaatgatt
cgcagtacattcagcccagatatagtaacagccatccttttgtaccctgtcgctggggagtcatttaaaatatggctggggacagtaaattaccgcattt
ggtttaacagtctttggccaaaggaaggccttcagcccaatttcccttcctccttaaacttagacccctggagggaaatttttccccccggggaaaaaaa
aaaaatttggggccccc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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