XB-CLONE-4351184
Clone Name: IMAGE:7026910
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Gene:
dcn
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785052 [+]
taattaatcccccccccccccgaaattacatctctgcttctagagttcttcttggaaatcccaggataacaaggataccatttgggctggatgaactaag
aagatgaaggcaactattatcctttttgtacttttgccactttgcatttctaaaccctttcaccagagaggcctgtttgactttatgctagaggatgatg
catctggggatgggtcatctctatggcctgaaccatctctacccactgatggaccaactggacctattggtcctatttgcccatttcgctgtcagtgcca
cctgcgggttgtgcagtattctgatataggtttggagcaagtaccaaaggatataccctccgataccactttacttgacttacagaacaacaagatcact
gaaatcaaggagggtgatttcaagaatctaaagaaccttcatgctttaattcttgttaataacaaaatcaagtcaatcagtccctctgcttttgcttctc
ttacaaaaatggaaaggctttacctatcaaaaaattaacctgaaagaccttcccagccaaccatgccaaaagtcctctcccaagaaacatttctggtttc
atggaaaaatttcaaattccccaaaaatttaaaaaaaaacctggtggtttcgaatgggaattaaaaataacccatggaattggtttggtatgaaaccgtg
gggtaacaaaattcccaaataaaaaaaaagttccagaggaaatttggaaaaaaggggaagccnttttccaaaggggaaatggaaaaaaaagcctgggtct
cttaaccctttccaaatttttggcggaataccttaaatattttactccaccttcattttccttgaaaaaggggcccttttcccagagcctttcgtatttt
aaactgtgatagcgtcttctccccccctttgtattgggcgcaaaaaaaaaaaaaga
3' EST: CF785051 [+]tttttttttttttttgattatccaatactactacaatttatttttttgaaaagtacagattatttggatcaacttttctgtgggtataatactaacagta
tacaataaacttaataagggaacagaacagcacagctaaatgtctttattattcatcattatgcaactcaaaacaaaataatgcagaaaactcctgtctc
atgatagtaagctggctattctgttttgggatactttccagtattggacatttttattcactaagaatgtatttaaacaaacaaacaaagaagagaaaac
aaacaaaaaaaagactttatttgcaacacgtccatgatttacttgtagtttccaatttgtatagcagatcgttcatatacacatctgaatgtggccggct
gaatttcccaatattgcactggattgctaaaaaggctgatgcctgagtatgaggccttctttgtgttatatccaagtgggcagaaatcattcgtggagac
agcagaaattttattgttatgaaggtacacaacctgaatgtacttgtgttcgccaagcccagcaggcacctgtgtcagggaattatggtctaagtgaagc
tccctttaatgaggcacacctgttactgtttcattttcaagagtaatgatgttgttataactgagacctaacttttgcaagttggttagtccattttaga
ctgtccgaatcaactttagccaaatttgtttccatcaaggtgaaactcanttaatgaaactggaaaggcctttagaatactgggta
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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