XB-CLONE-4351175
Clone Name: IMAGE:7026906
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Gene:
ndufs8
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785058 [+]
taattaatcccccccccccccgatctttgcaagagggactgtgaagatgttggttgtacgtctgatccaaaatgctgttaaacggccaggtgtttcttgt
actcagatgttgtcaaggcctctcagtctcgcttcacagatgcattcatataaatatgttaatgctcgagaggaggcaacagatctgaaatctgtgacgg
atcgtgcagcccagaccttactttggacagagctttttagaggcctaggaatgaccctgagttatctgttccgggagccagcaactattaattacccatt
tgaaaaaggtcctttaagtccacggttccgtggtgagcatgcattacgtaggtatccatccggtgaggagcgctgcattgcctgcaaactctgtgaggca
gtctgccctgcacaggctattacaattgaagcagagccacgtactgatggaagccgtcggactacaagatacgacattgacatgacaaaatgtatctatt
gtgggttctgccaggaggcatgtcctgtcgatgcaattgtggagggcccaaactttgagttcagcacagagactcatgaagaacttttatacaacaagga
gaaacttctgaataatggagacaagtgggaagttgagattggagcaaatatacaagctgactttctttaccgttgaaatctgtcccttcagctacttcag
aacactcaagccccatgaatagcggataactttgtgtgaagcggttatcatctctaacatggtcatccttttagtatgattacacttcagagtacttagt
taatatn
3' EST: CF785057 [+]tttttttttttttttaagggttaaaaacatgttttattttgcacattctatctattttcccagtttcatttttacactgaacaattcctttaattgctgc
catgaaagcacagatctctgtggattcaggatcatgcatattttaactacagcaaatcaactctaattaataacagcaagctgttatggagtacatgaag
acaatgaaatatatgtttcagattcagaaataatgtgatcatcttttacatgtgtacactcaatttaaaatgagaagtgtgcttgtttgttatataaaat
cacaggtggatctggcatgccagaaggtctttacaaataaggcagtgaagcaggataactcagaatggtgaaatgatagttaattctgagtagtgttttt
tagcacgtcaaaaacatagttgtccaccccaaccgtgttctgagccagcctgttcttgaagttccaggcattcttgcttagtacttagattaaagtaatt
aaagattacttttggaatttggaacacaagaaatttaatattcacaaagacaattgcacaaatgttcctctatttctttacaataattttccaaaatata
actaagtactctgaagtgtaatcatactaaaggatgaccatgttagagatgataacggctcacacaagttatccgctattcatggggcttgagtgttctg
aagtagctgaagggacagatttcaacggtaaagaaagtcagcttgtatattttgctccaatctcaacttcccac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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