XB-CLONE-4351166
Clone Name: IMAGE:7026903
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Gene:
atp1a1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC080463 [+]
GAGTGTCAGGCGGAGGGAGGCGAGCTCTACCGAGAAACGAAGAACAGACCGCCACCATGGGATACGGGGCCGGAAGAGACAAGTATGAGCCCGCAGCTAC
CTCTGAGCAGGGGGGCAAGAAGAAAAAGGGAAAGGGGAAGGATAAAGACATGGATGAGTTAAAGAAGGAATTGACCATGGAAGACCACAAACTGAGTCTG
GATGAGCTGCACCGCAAGTTTGGGACGGACATGCAAAGGGGTCTGAGCACGGCCCGGGCAGCAGAGATCCTGGCACGAGATGGACCCAATGCCCTCACCC
CGCCCCCCACTACTCCAGAGTGGGTGAAATTTTGCCGCCAGCTGTTCGGGGGGTTCTCTATGCTGCTGTGGATTGGAGCCATACTCTGCTTCCTGGCCTA
TGGTATCACCGCTGCCACGGAAGAGGAGCCAACGAATGATAATCTCTACCTCGGTGTTGTCTTGTCCGCTGTCGTTATCATCACCGGCTGCTTCTCTTAC
TACCAAGAGGCAAAGAGCTCTAAGATCATGGAGTCCTTCAAGAACATGGTTCCTCAGCAAGCCCTGGTAATCCGAAACGGAGAGAAGTTGAGTATTAATG
CGGAAGACGTGGTTTTGGGTGACCTGGTAGAAGTGAAGGGAGGAGATCGGATACCTGCGGATGTTCGGATCATCTCCGCTCATGGCTGCAAGGTGGATAA
CTCTTCCCTCACTGGAGAGTCCGAACCCCAGACCCGCTCTCCTGACTTCACCAATGAGAACCCACTGGAGACCCGCAACATCGCCTTCTTCTCTACAAAC
TGTGTGGAAGGCACAGCCCGTGGCGTTGTCGTCAACACCGGTGATCGTACAGTGATGGGCCGTATCGCTACTCTGGCCTCTGGCCTGGAGGGAGGACGCA
CCCCCATTGCCATTGAAATTGAGCATTTTATTCACATCATCACCGGCGTGGCGGTCTTCCTGGGGGTCAGCTTCTTCATCCTGTCACTCATTCTGCAGTA
CACCTGGCTGGAGGCTGTCATCTTCCTCATTGGTATCATTGTTGCCAATGTGCCAGAGGGACTGCTGGCTACTGTCACGGTTTGCCTTACACTGACCGCC
AAAAGAATGGCCCGCAAGAACTGTCTTGTGAAGAATCTGGAGGCTGTGGAGACTTTGGGATCCACTTCCACCATTTGCTCCGACAAAACGGGCACCCTGA
CACAGAACCGAATGACCGTAGCCCACATGTGGTTTGATAACCAGATCCATGAAGCCGACACCACGGAGAATCAGAGCGGTGCCTCCTTCGATAAGAGCTC
CCCCACATGGACAGCCCTGTCCCGGGTTGCTGGATTGTGCAACAGAGCCGTGTTTCAGGCTGGACAAGAGAACACCCCCATCCTAAAGAGAGATGTTGCT
GGAGATGCATCCGAATCCGCCCTGCTAAAGTGCATAGAACTCTGCTGTGGGTCTGTGAGGGATATGAGGGAAAAGAACCCCAAAGTGGCAGAAATTCCAT
TTAACTCTACAAACAAATACCAGCTGTCCGTACACAAGAATGCAAACCCATCAGAGTCCCGTTACATTCTGGTCATGAAAGGGGCCCCCGAGCGTATCCT
GGACCGATGCTCCACCATTGTGCTGCAGGGCAAGGAGCAGCCCTTGGACGAGGAACTGAAGGATGCTTTCCAGAATGCCTACCTGGAGCTGGGGGGCCTG
GGAGAGAGAGTGTTGGGTTTCTGCCACCTTGCTTTGTCCGATGACCAGTTCCCTGATGGATTTCAGTTTGACACAGAGGAAGTGAACTTCCCAACAGAGA
ATCTTTGCTTCGTTGGGCTGATTTCCATGATTGACCCGCCCCGTGCCGCTGTGCCTGATGCCGTCGGCAAATGCCGAAGTGCTGGAATCAAGGTTATCAT
GGTGACGGGTGACCACCCAATCACAGCCAAGGCCATTGCTAAGGGAGTGGGAATCATCTCTGAAGGCAATGAAACAGTGGAGGATATTGCTGCCCGCCTC
AACATTCCAGTCAACCAGGTGAACCCCAGAGATGCCAAGGCCTGTGTGATCCACGGGTCGGACCTGAAGGACATGGCACAAGAACAGATCGATGACATCC
TGAAGCATCACACAGAGATAGTCTTTGCCAGAACATCCCCTCAGCAGAAGCTCATCATTGTGGAGGGTTGTCAGCGACAGGGTGCCATTGTGGCAGTGAC
AGGGGATGGTGTCAACGACTCCCCTGCTCTGAAGAAGGCAGATATTGGTATCGCTATGGGCATCGCTGGTTCCGATGTGTCCAAGCAGGCAGCCGACATG
ATCCTGTTGGATGATAACTTTGCTTCTATCGTTACTGGAGTAGAGGAAGGTCGTCTGATCTTTGATAACCTGAAGAAATCCATCGCCTACACCCTGACCA
GTAACATCCCAGAAATCACACCCTTCCTCATCTTCATCGTCGCCAACATTCCTCTGCCCTTGGGCACAGTCACCATCCTGTGTATCGATCTGGGCACAGA
CATGGTCCCCGCTATCTCCCTGGCCTATGAGCAAGCCGAGAGTGACATCATGAAGAGACAGCCCCGTAATCCCAAAACAGACAAGCTGGTGAATGAAAGG
CTTATCAGTATGGCCTATGGGCAGATCGGTATGATCCAAGCCCTGGGGGGGTTCTTCACATACTTTGTGATCCTGGCTGAGAATGGCTTTCTGCCCTGGA
CACTGCTGGGTATCAGAGTGAACTGGGACGACCGCTGGAATAACGACGTGGAGGACAGTTACGGGCAGCAGTGGACCTACGAGCAGAGGAAGATTGTGGA
GTTCACGTGCCATACGGCTTTCTTCGTCAGTATCGTGGTGGTGCAGTGGGCCGATCTGATCATCTGCAAGACCAGAAGGAATTCAGTGTTCCAGCAGGGC
ATGAAGAATAAAATCCTTATCTTTGGGCTGTTTGAAGAGACTGCACTGGCTGCCTTCCTGTCCTACTGCCCTGGCATGGATGTGGCATTGCGCATGTACC
CACTCAAGCCGACCTGGTGGTTCTGTGCCTTCCCATACTCGCTGCTCATCTTCATATACGATGAAGTCCGTAAGCTGATTATCCGGCGCAGCCCAGGCGG
CTGGGTGGAGAAAGAAACCTACTATTAGAGCCTTCGGGTCGTCCGAACATTTCACGTCACTTCCTGCCCCTCGGTCTGCCTGCACCCACCCGTCGCCTGC
GTGCACCCGTCTGACTCAGCCTGTTCCCATGGAAGAGATGCATTTAGGCAAGGGGCGCAGTGTCGCCTGTGTGCGTCCTGTACAGAGTTCTCATTGTGCC
TTCTCCGGTATTGTTTTTCGTTTGTTTCTTTTTTTTGTTTCTTTTTGTAATGGGAGCACGTTCAGAGATGTTTTATATATCTGCATTTTTACAAATAAAG
GCGGTTTGAAGAGTAAAAAAAAAAAAAAAA
5' EST: CF785064 [+]taattaatcccccccccccccgagtgtcaggcggagggaggcgagctctaccgagaaacgaagaacagaccgccaccatgggatacggggccggaagaga
caagtatgagcccgcagctacctctgagcaggggggcaagaagaaaaagggaaaggggaaggataaagacatggatgagttaaagaaggaattgaccatg
gaagaccacaaactgagtctggatgagctgcaccgcaagtttgggacggacatgcaaaggggtctgagcacggcccgggcagcagagatcctggcacgag
atggacccaatgccctcaccccgccccccactactccagagtgggtgaaattttgccgccagctgttcggggggttctctatgctgctgtggattggagc
catactctgcttcctggcctatggtatcaccgctgccacggaagaggagccaacgaatgataatctctacctcggtgttgtcttgtccgctgtcgttatc
atcaccggctgcttctcttactaccaagaggcaaagagctctaagatcatggagtccttncagaacatggttcctcagcaagccctggtaatccgaaacg
gagagaagttgagtatttatgcggaagacgtggttttgggtgacctggtagaagtgaaaggaggagatcggataccctgcgatgttcggatcatctccgc
tcatggctgcaaggtggataactcttccctcacgggagagtccgaaccccagacccgctctcctgacttcaccaatggagaaccccacgggaaaacccgc
caaca
3' EST: CF785063 [+]tttttttttttttttactcttcaaaccgcctttatttgtaaaaatgcagatatataaaacatctctgaacgtgctcccattacaaaaagaaacaaaaaaa
agaaacaaacgaaaaacaataccggagaaggcacaatgagaactctgtacaggacgcacacaggcgacactgcgccccttgcctaaatgcatctcttcca
tgggaacaggctgagtcagacgggtgcacgcaggcgacgggtgggtgcaggcagaccgaggggcaggaagtgacgtgaaatgttcggacgacccgaaggc
tctaatagtaggtttctttctccacccagccgcctgggctgcgccggataatcagcttacggacttcatcgtatatgaagatgagcagcgagtatgggaa
ggcacagaaccaccaggtcggcttgagtgggtacatgcgcaatgccacatccatgccagggcagtaggacaggaaggcagccagtgcagtctcttcaaac
agcccaaagataaggattttattcttcatgccctgctggaacactgaattccttctggtcttgcagatgatcagatcggcccactgcaccaccacgatac
tgacgaagaaagccgtatggcacgtgaactccacaatcttcctctgctcgtaggtccactgctgcccgtaactgtcctccacgtcgttattccagcggtc
gtcccagttcactctgatacccagcagtgtccagggcagaaagccattctcagccaggatcacaaagtatgtgaagaaccccccccagggctggaatcat
accgatctgccccataggccatactggataagcc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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