XB-CLONE-4351155
Clone Name: IMAGE:7026898
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Gene:
rps8
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785071 [+]
taattaatccccccccccccccgcctctttccagccagcgcctgagaatgggtatctcaagggacaactggcacaagcgccgcaagactggtggcaaaag
gaagccttatcacaaaaagagaaagtatgaacttggccgtcctgctgcaaacaccaagattggtcctcgccgtatccacactgtgagagtccgcggtggt
aacaaaaagtatcgtgctctgaggttggatgctggcaacttttcatggggttcagagtgttgcacacgtaaaactaggatcattgatgtggtgtacaatg
cgtcaaacaatgagctggttcgaaccaaaacccttgtgaagaactgcattgtcctggttgacagcactccttacagacagtggtttgaggcccactatgc
tctcccactaggacgcaagaagggagctaaactcacccctgaagaggaagaaatcctaaacaagaaacgatctaagaaaacacagaagaaatatgaggag
aggaaaaagacagcaaaaataagcccacttttggaagaacagttccagcaaggcaaactgcttgcatgcatagcctccagaccgggacagtgtggtagag
ctgatggttatgtactggaanggaaggagttggagttctacctgagaaagatcaaagccaaaaaggggaaataaacatgttaccaccacttatcatcaaa
ttaaatgtttattaaaaacaaaaaaaaaaaaaaaaagggatccccttaatttcttagaggcggcccgcccaccggcggtgggaacctttggcccgttggg
cccaaactttggttttattggcacg
3' EST: CF785070 [+]tttttttttttttttgttttaataacatttatttgatgataagtgtggtaacatgtttatttcccctttttggccttgatctttctcaggtagaactcca
actccttcccttccagtacataaccatcagctctaccacactgtcccggtctggaggctatgcatgcaagcagtttgccttgctggaactgttcttccaa
aagtgggcttatttttgctgtctttttcctctcctcatatttcttctgtgttttcttagatcgtttcttgtttaggatttcttcctcttcaggggtgagt
ttagctcccttcttgcgtcctagtgggagagcatagtgggcctcaaaccactgtctgtaaggagtgctgtcaaccaggacaatgcagttcttcacaaggg
ttttggttcgaaccagctcattgtttgacgcattgtacaccacatcaatgatcctagttttacgtgtgcaacactctgaaccccatgaaaagttgccagc
atccaacctcagagcacgatactttttgttaccaccgcggactctcacagtgtggatacggcgaggaccaatcttggtgtttgcagcaggacggccaagt
tcatactttctctttttgggaaaaggcttccttttgccaccagtcttgcggcgcttgtgccagttgtcccttgagatacccattcctcagcgctggctgg
aaagaggccgn
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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