XB-CLONE-4351152
Clone Name: IMAGE:7026897
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Gene:
prmt1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785073 [+]
ttaatcccccccccccccggcagttctctcgtacaaagccggtggcgtggctttgagtttcttttttagattcgtgagagagactgacaaacatggctga
agcgagcacctgcaacatggaggtttcttgtactcagcctgagagcagtgtgaagccaaatgcggaggacatgacatcaaaagattactactttgactcg
tatgctcactttggcatccatgaggagatgttaaaagatgaagttcgaacgctgacatatcgtaactcaatgtttcacaacagacatctatttaaagata
aagtagtcttggatgttgggagtggcacgggtattctctgcatgtttgcagcaaaggctggtgccaagaaagttattgggattgagtgctcaagtatatc
tgactatgccataaaaatcgtgaaggcaaataaactagatcatgtggttacaatcatcaaaggcaaagtagaagaggtggagctcccagtagaaaaagtg
gacatcataatcagtgaatggatgggatattgtctgttctatgaatcaatgctgaacactgttatctatgcacgggataaatggctgactccagatgggt
taatatttccagatcgtgctacactgtatgtaactgctattgaggatagacaatacaaagactaccaaattcactgggtgggaaaaacgtttatggcttt
tgatatgtcatggcattcagggatgtagcccatcaagggaggcctcctgggttgatgtttgttggaatccaaaacaaactgggtcacttc
3' EST: CF785072 [+]tttttttttttttttgcagttcactttttaatttcattatgaatgcaaccatataaaaaccataagttatgaaacagtcacaatgggaagcttgaaggat
caataaaacaaatgaagggcctttccataccaaggtctgagaggacttacaaaaacagtacaaaacacccaagccaagtgagcaacccttttgttcctca
ataggatgggtgcctgctcctcataagggctagacaagggatactggtgataccatacacattgcaacaggacagaaaacccaccctgatagtgtccatt
agttaaaaaaaaaaaaaaaaaagtcctgcaaaaaaaaagtgaaagggaatgcacactttggagcttaacactttgctgccagcaggggctgaatactttt
gcagtgtatacccctttaggccactcagtaatcaagtaatgggtttcctattgttaaaatagtctgcaattctagcaattcattgactgaatatactaaa
aacaaaaaggaatgcctcttgggatagacctgctaaaggcaggaaaacttanggggctcggaaactgaacagatggtttgaatccattatggatctttta
ttacatgatgaacccagccaagggagctggatcaatactctagccttaatggccacagtggnaggaacaagatcatgggcttttggttttccccaaagga
acctccttgcactcctaacactgaaattggggctttctaaaaatggtttcccc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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