XB-CLONE-4351146
Clone Name: IMAGE:7026895
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Gene:
otub1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785077 [+]
taattaatcccccccccccccgtggaaatatggcggcggaagaatctgagcggcagcagcaaagtgattgtgacggtgtgaactgtcttacttacgatga
agcaattattgctcagcaggatcgtattcagcaggagattgctggtcatactcctttgctctctgaacgtttggacctgtctgtactgtaccaagagtat
gcccctgatgaccaggtgtaccaagaaaagatcaaggacttacatagaagatataatcacatacgacgtacacggccagatgggaactgtttctatcgag
cgtttggcttcgcttatttagaagctttgcttggaggaggcccagagcttgcaaggttcaaggaggtgtcgttacaaagtaaggatgaactgataaggca
gggatttacagagtttactatagaagatttccacaatacgtttatggaacttattgcgcttgtcgaaaagcgtcctgctgttactgagctcttagctgca
tttaatgagcagactgtatcggactatgtggtggtgtatttacgtctcctgacatctggtcacctgcaaaaagacagcatcttctaccagcattttatag
agggaggaagaagtgtcaaagaattctgccagcaggaggtggagcccatgtgtaaggaaagtgaccacatacacatcatatctctgtcccaggccctaaa
tgtctccatccaggttgaatatatggacagggggtgagggggggaaccacagtcacccatgtttttcccctgaaggttcagatcccccctcaggtctttt
ctacc
3' EST: CF785076 [+]tttttttttttttttggattttattatttatttttctccgtagagataaaacgtaacagagaggggagatgagggaaagccgagaccaggcgaggggggt
agggagggggggagagaacagttttgcagtaaggaatttacagaacagtgcagaatcaggtcacttgtagagaatgtcgtagtgtccaggtcgatatagt
agaaagacctgagggggatctgaaccttcagggaaaacatgggtgactgtggttcccccctcacccctgtccatatattcaacctggatggagacattta
gggcctgggacagagatatgatgtgtatgtggtcactttccttacacatgggctccacctcctgctggcagaattctttgacacttcttcctccctctat
aaaatgctggtagaagatgctgtctttttgcaggtgaccagatgtcaggagacgtaaatacaccaccacatagtccgatacagtctgctcattaaatgca
gctaagagctcagtaacagcaggacgcttttcgacaagcgcaataagttccataaacgtattgtggaaatcttctatagtaaactctgtaaatccctgcc
ttatcagttcatccttactttgtaacgacacctccttgaaccttgcaagctctgggcctcctccaagcaaagcttctaaataagcgaagccaaacgctcg
atagaaacagttcccatctggcccgtgtacctcggaatgtgaatatatccttcaaggtaagttctttgatccttttcttggtacaccctggtcatcaggg
ggcattattccttggtacaatt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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