XB-CLONE-4351132
Clone Name: IMAGE:7026890
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Gene:
ddx39a
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785086 [+]
taattaatccccccccccccccggcacacgtggagcaggagccgcagcacagaaagagccatcatgacagaacaagacgtagaaaatgagctgttggatt
acgaagaagacgacgagcctcaggcacccgctgaaactgcagctccaatcgcccggaaagaggtgaaaggctcctacgtgtccatccacagctccggctt
ccgagacttcttgctgaaacccgaactcctccgatccatcgtagactgcggttttgaacatccctctgaggtccaacatgagtgtatccctcaggctatc
ctgggcatggacatcctttgccaagcaaaatctggtatggggaagactgctgtctttgtcctggccaccctgcagcaaaccgaagccgtggaagggcagg
tgactgtcctagttatgtgccacacgcgtgagctagcgttccagatcagcaaggagtatgagcggttctccaaatacatgccgactgtcaaagtggcagt
tttctttggcgggctctctattaagaaagatgaagacaccatacgcaagagctgtccccatattgttgtcggaacaccgggtcgcatcctggcgctcgtc
agaagcaagatcctgaatctgaagaacgtgaaacactttgtgttggacgagtgtgacaagatgctggagcagttagatatgagaagagatgtacaggaga
tttttcgccctacaccccatgagaaacagtgcatgatgttcagtgccacccttgagtaaagagatccgcccctgtgtgccgggaagttcatggcagatcc
tatgggagg
3' EST: CF785085 [+]ttttttttttttttaataataaaaaagtttaatctttttacccttctttaaacaaaaacaacaaacatttcataaaaaaacccaaacagtaaaaccgaag
ccgccattccctgcacgggtgcgtgggcagacgaagggagcagaatggaggcgggcgccacagtcaccttgggagagggggcaacacggacgaggtggtt
atcgggtctgctcaatgtaagtagatatgtctatttcatcaggcagctcccccacgttgacttcaaacctgtcctgaacgtcattaaggattttggcatc
ttcctcatccgacacaaaggttatggccaagccctttgtaccaaatctgccggcacgggctaccctgtgcaggtatgtgtcagagtcttctggcatgtcg
taattaaacacaatattcactcgctcgatgtccattcctcgaccaaacagatttgtggccaccaggattcttctctgaaagtctttaaattgctggtatc
tggagagcctctcctcctgcgacatgtttctgtgaatagcgatagcagggaagttttgttcagtcaagagctgggccagagccatgcatctctgcactga
tttcacaaagatcaccacctgattaaactctaatacgtccagtagatcaaaaagcttgcggttcttctcactgtcttttaacttgacataatattgctgc
aggccatgaagggtcagcttggtctcatcatccacaaatacctccataggatcttgcatgaacttccggcacacagggcggatctctttactcaggntgg
cactgaacatcatgcactgtttctc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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