XB-CLONE-4351129
Clone Name: IMAGE:7026889
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Gene:
dync1h1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785088 [+]
taattaatccccccccccccccccccctttcttggatatttaccatacagtactgtatgagaacgctaatctgaagggaaccatagatcatactcagcgc
ctctctgtcatcaccaaggaccttttctaggttgctttcaatagagttgcccggggtatgcttcaccaggaccacataacctttgccatgcttcttgcaa
gaataaaactaaaaggaactattggggagcctgcatatgaccttgaatttcaacatttcctaagaggaaagcagattgtgcttggttcaacttttgtacc
caaaatcagtggattaattgaatagcagttaaaagccatgatgcgacttagcaacttgtctgcatttacggatgtgatctcaaaggtcgaggctgatgaa
caattctggctatggtcagacagcagttctcccgaattaaccgtgccctcgattttggagtgaagaataattctctgccaacccaaattggtacaaaact
gttgttaaccggcttaggttggtatcaataccaaagtcttcttccagctcctaggatttaggttattcccctgtgcgccgtggggcaaccattacttttg
gatgggttcccctacaccattttttccacacggcgaagaatatttgctctatttgaatgggtgccacatctatattgatgcaacacatacgccccttata
tgtgaacactcgggggcgggtagtttttgttggatcacccactccccgtgaaggggttgaatgtaactgttaaatattcaccgccgttgtcttcctcacc
acgctgtttttgttgctcaacccttaccgttatatttgtattccctcccgggggtactgtatttttctcacataatttttgtgccagcctcttaatttaa
caaactacatttccctaataaaacgtcctaggtgaacccattgaggggagcaatggtgaaaaaaggggtcgttttttcttttgtattttttacacacgga
ttcgatactttttttcttcatccaaagg
3' EST: CF785087 [+]ttatgttttccactgtacgttttagatgattgagtgcttgtgggatgacttgaagccagttgcaggcagttatgtgtaaagttctcatccaggctgggcg
gccgtcagatgtggagtctgttctttgtttcttctcagtttctgcataagctaagtcatcctcatcctccagcatttgcattttcagcattttaccgatc
atgtcaatgccctgtgttgtgagaagaactctttctgcattgttaggcagtccaagccaggagggtgtctgggtatctgggagagtctccacccactgaa
caaattcatcacgcctgatgccatctggcatttggatatccttgtgcccatcaaccttacaagctaatttaaactcgctgtcaaaacttgaagttgtaaa
gagtcgttcaaggaatgtgtttagtagcctttggtcaaattcattatcaatccgccctccataaatagactgtgccatcaaagtttttagggctgaccat
ggaattttatctggtgaaatgttctgacggcccttcgcagtgtcatcaagccatgtgtccacagtgtcacaagcagatctgagatccgattcaccaaatt
catacttcttagaccagcccaaaggagcatagcgcagacgttcttggataatagcatgaaaccaggccaggaggaagtataatcgagcacgctcattggg
agatttacaaatcctcgaaacagggatactactgaatgttctcagcatgttggcctttacaccangtggtggttcanagacaaagatc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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