XB-CLONE-4351112
Clone Name: IMAGE:7026883
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Gene:
ubox5
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785099 [+]
taattaatcccgcccccccccgtaactcagatgcagcatcccatgagcaaaggctgacccaaagcctggactacgcactcactactacacttggatccat
gccctcgtacactgcaaggtttgtgaaaggccaccaactgggagcttcaggggagagcggtggaggaacctcggcctggagcaccatacccgagtttaat
cgatgtgccgagggtcaaggttgctcttcttgtgggaggatattttccgcttactgcaagacagagcccgtgtatcagctttcatgtgggcacctcatgt
gccggccttgcttagcggagaaacaaaagtccctgtccattctgtgcaacaattgcaacaggtcggtggccacgcgagatgttcagagagtccacctttg
atttgcacaatgaataaaggctttgcccaggaaacggctgaaacactgcattcagatgactgggcctgctccgaggccttgatacgtaccgttaacttta
tatcctttgctgctattctcttgtgtatttatttaggagcggggggcagtagtgaccccactattctgcttaataccatagctgtgctagctggaggaat
tcactcagacctattcatgttatgtttacagtggataaccctagggccggttcgaaggtgggattgattacccacaggtttcactctctcaatctaagta
atagatggcctcttcttacccccaggcccctggtgaatccctattcatgtgcaaagtaacaacaaaggtcttttacattttgtggttgggaataaagttg
cactttntggaaa
3' EST: CF785098 [+]tttttttttttttttcacaagtgcactttattccaacacaaatgtaaagaccttgttgttacttgcacatgaataggattcaccagggcctgggggtaag
aagaggccatctattacttagattgagagagtgaaacctgtgggtaatcaatcccaccttcgaaccggccctagggttatccactgtaaacataacatga
ataggtctgagtgaattcctccagctagcacagctatggtattaagcagaatagtggggtcactactgccccccgctcctaaataaatacacaagagaat
agcagcaaaggatataaagttaacggtacgtatcaaggcctcggagcaggcccagtcatctgaatgcagtgtttcagccgtttcctgggcaaagccttta
ttcattgtgcaaatcaaaggtggactctctgaacatctcgcgtggccaccgacctggtgcaattgttgcacagaatggacaggggactttttgtttcctc
cgctaaacaaagcccggcacatgaagtgccccacatgaaagcctgaatacaccgggctctggtcttgccataagccgaaaaatattcctcccccaagaag
gagccaacccttggacccctcgggcacattcgaatttaaactccggggtatggtggcttccagggcccgaaggttcccttccaaccggttcttcccccgg
aaacccttcccatttttgttgggcctttttcacaaaaccctttgccagttgtaccaaaggggcatggggattcca
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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