XB-CLONE-4351094
Clone Name: IMAGE:7026876
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Gene:
upk2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785110 [+]
taattaatcccccccccccccgtggcagttctaggcattgtagaagcaggttggaaacaagtgcagtcacagagagacatcagcatgcagctcctgggga
tcatcaccgtgctacttctcatctctggtgccatagctcagaatgtttcccaggcgacaggggttcttactccacttgctacatctgctatatttgcttt
tccagactgcacttactcaggacagactgttagcttggtcataactaacagcacatctactgctactattatacaaaatgctaccttccaggtaccacag
tgccgccttaaacgtgatattgttgtgattaataatggacagtccggtaatgtgcagactgtcaatgtgggctaccagatccaaaacctacagccaggta
ccaactacatggcgacatattcaagtggtggaatctctggaccatccttccaatttagtacaagaacagtgtatccagctgtcgcaaatataatggctcg
cagtggaggcatggtggtgatcactgtcttgttaagcattgcgatgttcgtgctcttggctgggctaatcgctgttcttattcttggaaggaaatgagta
cagaaaggggatggccttggagggctaacagatttctgtgtcctttttaatggatgttcatctaacattaaaaatatttttatgtttatcttaaatagaa
tagtatgcatattcatcttgagcaatagcaaaaccaaataatttgtgtaaataattttttt
3' EST: CF785109 [+]tttttttttttttttatggataataaacatttattacaaataaaccactgttcaattaaaacacataacagtttgtatacattatcaagaatccccgcat
acatggtggcacattgattcaccaaacaccagtccattctactttgccactctcccaccacagtgctgaaaggtggagagttcaattctataaaaatcat
ggtaaaaaaaaactatttacacaaattattttggttttgctattgctcaagatgaatatgcatactattctatttaagataaacataaaaatatttttaa
tgttagatgaacatccattaaaaaggacacagaaatctgttagccctccaaggccatcccctttctgtactcatttccttccaagaataagaacagcgat
tagcccagccaagagcacgaacatcgcaatgcttaacaagacagtgatcaccaccatgcctccactgcgagccattatatttgcgacagctggatacact
gttcttgtactaaattggaaggatggtccagagattccaccacttgaatatgtcgccatgtagttggtacctggctgtaagttttggatctggtagccca
cattgacagtctgcacattaccggactgtccattattaatcacaacaatatcacgtttaaagcggcactgtggtacctggaaagtagcattttgtataat
aataacagtaaatgtgctgttagttatgaccaaactaacagtctggtcctgagtaaatgcaagtctgggaaaaccaaattatgcccaaagttaccaagtt
ggagttaagaaaaccccca
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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