XB-CLONE-4351091
Clone Name: IMAGE:7026875
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Gene:
rpl35
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077011 [+]
GCCCTTTCCTCTGGCGGCCATCAGCGATAATGGCAAAGATCAAGGCCCGAGACTTGCGTGGTAAGAAGAAGGAAGAGCTGCTGAAGCAGCTCGATGACCT
CAAAGTAGAACTGTCACAGCTCAGAGTTGCTAAAGTGACAGGAGGTGCTGCTTCAAAGCTGTCTAAGATCCGAGTTGTGCGCAAGTCTATTGCCAGAGTT
CTGACTGTGATCAACCAGACACAGAAGGAAAACCTGAGGAAATTTTACAAAGGCAAGAAATACAAGCCTTTGGATCTGAGGCCAAAGAAGACCAGGGCCC
TTAGGCGCCGGCTAAACAAGCATGAAGAGGGTCTGAGGACAAAGAAACAGCAAAGGAAAGATCGTCTTTTCTCTGCACGCAAATTTGCTGTCAAGGCTTA
AGTTTCATGTTCTAAATAAAAAAAACTGTTTGGATTGGCCAAAAAAAAAAAAAAAA
5' EST: CF785112 [+]taattaatcccccccccccccgccctttcctctggcggccatcagcgataatggcaaagatcaaggcccgagacttgcgtggtaagaagaaggaagagct
gctgaagcagctcgatgacctcaaagtagaactgtcacagctcagagttgctaaagtgacaggaggtgctgcttcaaagctgtctaagatccgagttgtg
cgcaagtctattgccagagttctgactgtgatcaaccagacacagaaggaaaacctgaggaaattttacaaaggcaagaaatacaagcctttggatctga
ggccaaagaagaccagggcccttaggcgccggctaaacaagcatgaagagggtctgaggacaaagaaacagcaaaggaaagatcgtcttttctctgcacg
caaatttgctgtcaaggcttaagtttcatgttctaaataaaaaaaactgtttggattggccaaaaaaaaaaaaaaaag
3' EST: CF785111 [+]tttttttttttttttggccaatccaaacagttttttttatttagaacatgaaacttaagccttgacagcaaatttgcgtgcagagaaaagacgatctttc
ctttgctgtttctttgtcctcagaccctcttcatgcttgtttagccggcgcctaagggccctggtcttctttggcctcagatccaaaggcttgtatttct
tgcctttgtaaaatttcctcaggttttccttctgtgtctggttgatcacagtcagaactctgggcaatagacttggcgcaccaactcggaattctagaca
gcttttgaacaccacctcctgtcactttagcaactctgagctgtgacagttctacctttgaggtcattaagctgcttcagcagcccttccttcttcttaa
ccagaaagttttcggggcctggatttttggccatttttcccgaaaggcccccccaaaaagaaaagggcggggggggggggggggaaattaaattaaaccc
cggggggcaaaaaaaaaaaaaagaaaccttgggatttttggggccccggaaaaaaaccccaaagggggccattttgggccccccggggaggggggaacac
cccttggggggaaaaaaaaagggccaaagggggggtttttttttttctttaaaggggggcccccacaatag
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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