XB-CLONE-4351085
Clone Name: IMAGE:7026873
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Gene:
elp6
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC080461 [+]
GTGCTTGTCAGACATGTCTGCCTTCGGCCGCTAGTTACTTAGTTGAACTAATACCTGTACCCACAGATACTACTGATAATAATAATAATAAGCACATTTA
ATGGTGAGTTATTGCGCCTCAAACTGCTTCACTGACGCGGAAGCCGTACTCCTGCCGCGAAACCATCTCCATGACAACCAGGTGTGGCGGGTTCCTGGCG
GCCTAGTGTCCTGTAGCGACAGTGAGAGAAGAGCGGGGATTGGAGGATGTTTCCCGAGCTAAACAGCCTGCTGGGAGCCGGCACTGATACAGTGGAACGG
GGCAAATTTACTCTCCTCTGTGACTCCAAGACCGATGGGAGCTTCCTGGTCCATCACTTCCTGTCCTACTACCTGAGGGCGGGGTGCAGGGTGTGCTTTG
TGGCCCTGGTGCAGTCATTCAGTCACTACAGCATCGTTGCCCAGAAGCTGGGAGTGAACCTGAGCTCGGCTAAGGACGAGGGGCAACTGGTGTTCCTGGA
AGGGCTGAGATCGTACACGGACTTGCTCTTTGGTGACAATCCGGAGGCAGAAGTAACCAACCCTCTCTGTTTCCTAAGGGCAGGATCAGACCTGAAACCT
TTATACAGCTTTGTTAGTGCTGCACTGGCGCCATCTGCTGGTCAGTCGTGGAAGTGTCCTGTGCTGATCCTGGATGATGTGAGTGTCCTGCTCAGTCTGG
GGGTGCCCCCCCTGCAGCTGCTGGACTTCATGCATTACTGCAGGGCCACTGTGTGCACTCAGTACCAGGGTAACGTGGTCTGCCTGGTGCATGGAGCGGA
GGAGTCGGGGGATGAGGAGAAGGAGCTGCTGCGGAGGTCTCTGTCCCACCAGAGCCAAGTGATCCTGTGGGCGGAAGGGCTGAGCTCGGGGTTCTGTAAG
GAGGTACATGGGCAGCTGAAGATTTTCAGGGGGGCCGTGTCCAGTGGGAAAAAACGGGGGCAAACCCAGCCGGAGATCTACCAGTACAAGATCCAGGACA
AACACGTCACGTTTTTCGCCAGAGGGCTGTCGGCGGCTGTGCTCTGAGTGAGCCCCAAGGGCTGCTGGGATTGGGACTGTGCTGGCAGAGTGCTCCCCCT
GCTGGTTATGTATGGTACGTGCCTTGTACATGCGTGTCCTGCTCCTATTGGCCTGGGTAGGGTCAGCTGACCCACAGCCACACAGTACAGATCCAGTTCT
CTGCCTGCAGGAAGTGCAAGATAAATACGCCCAGGCCTAGAGTGTCAGCCCCCGCGTACCGGCCAGGCCTTCCTTTCCTTTAGGCCCACTAGAGTGTCAG
CCCCCGCGTACCGGCCAGGCCTTCCTTTCCTTTAGGCCCACTAGAGTGTCAGCCCCCGCGTACCGGCCAGGCCTTCCTTTCCTTTTGGCCCACTAGAGTG
TCGGCCCCCGCGTACCGGCCAGGCCTTCCTTTCCTTTAGGCCCACTAGAGTGTCAGCCCCCGCGTACCGGCCAGGCCTTCCTTTCCTTTAGGCCCCCGGC
ACCGGTCTGAGTGGGCAGTGTTACTAAATTCATACACTTTTGCCCTTGTTTTATAAAAATGCCCAGGTCACCCTAGTAGTTGGCTCCATCATGGTGGCAC
TGGAAGCCCATTGGCCGGCACCCACACTGAGGGGCTTATTTACAATGTTGGTTAGATGCGAACTTGCATGTTACTGCAAATACTATTTTGCTGAAAATGT
ATAATTTGTAATGTGTTTCTGAGCATTTGGGATTATTCCAACCTGCCTATAAACTATTTCACTAAAAAAAAAAAAAAAA
5' EST: CF785116 [+]taattaatcccccccccccccgtgcttgtcagacatgtctgccttcggccgctagttacttagttgaactaatacctgtacccacagatactactgataa
taataataataagcacatttaatggtgagttattgcgcctcaaactgcttcactgacgcggaagccgtactcctgccgcgaaaccatctccatgacaacc
aggtgtggcgggttcctggcggcctagtgtcctgtagcgacagtgagagaagagcggggattggaggatgtttcccgagctaaacagcctgctgggagcc
ggcactgatacagtggaacggggcaaatttactctcctctgtgactccaagaccgatgggagcttcctggtccatcacttcctgtcctactacctgaggg
cggggtgcagggtgtgctttgtggccctggtgcagtcattcagtcactacagcatcgttgcccagaagctgggagtgaacctgagctcggctaaggacga
ggggcaactggtgttcctggaagggctgagatcgtacacggacttgctctttggtgacaatccggaggcagaagtaaccaacccntctctgtttcctaan
ggcaggatcagacctgaaacctttatacagctttgttagtgctgcactggcgccatctgctggtcagtcgtggaagtgtcctgtgctgatcctggatgat
gtgagtgtcctgctcaatctgggggtgcccccccctgcagctgctgggacttcctggattactgcagggccacttgtgttgccttcattac
3' EST: CF785115 [+]tttttttttttttttagtgaaatagtttataggcaggttggaataatcccaaatgctcagaaacacattacaaattatacattttcagcaaaatagtatt
tgcagtaacatgcaagttcgcatctaaccaacattgtaaataagcccctcagtgtgggtgccggccaatgggcttccagtgccaccatgatggagccaac
tactagggtgacctgggcatttttataaaacaagggcaaaagtgtatgaatttagtaacactgcccactcagaccggtgccgggggcctaaaggaaagga
aggcctggccggtacgcgggggctgacactctagtgggcctaaaggaaaggaaggcctggccggtacgcgggggccgacactctagtgggccaaaaggaa
aggaaggcctggccggtacgcgggggctgacactctagtgggcctaaaggaaaggaaggcctggccggtacgcgggggctgacactctagtgggcctaaa
ggaaaggaaggcctggccggtacgcgggggctgacactctaggcctgggcgtatttatcttgcacttcctgcaggcagagaactggatctgtactgtgtg
gctgtgggtcagctgaccctacccaggccaataggagcaggacacgcatgtacaaggcacgtaccatacataaccagcagggggagcactctgccagcac
agtcccaatcccagcagcccttggggctcactcagagcacagccgccgacagccctctgggcaaaaaacgtgacgggtttgtcctggatcttgg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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