XB-CLONE-4351064
Clone Name: IMAGE:7026865
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Gene:
ewsr1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785129 [+]
taattaatcccccccccccccgacacaaaatggcgtctaccgattacagcacctatagccaaactggagcccaacaaagttacagtgcatatactgcaca
accaactcaaggctacgcacagactgcacaggcaacttatgggcagcagagttatggcacctatgcacagcccactgatgccagttacacacaagcacag
acaacagccagctatggccagtctgcctatgccacttcttatggacagccaccaacaggatacactgctccagctgctccccaagcatatagccagccca
tgcagggatacggctctacaggctatgacagtaccactgcaacaactaccacaactcaggctacatatgctgcaccctctgcctacggttctcaacctgc
atatccttcttatggtcagcagcctgccactactgcatctgcaagacctcaagatggaagcaaaccagcagataccagtcagcccccaccaagtacagcc
tcatatgcccagcccagcatgggatatagtcaaagcagctacagctaccctcaagttccagcaagctatccaatgcagcaagtgagcgcccctccatcct
accctcctacaagctactcgagctctcaaacatcaagctacgagcagagctcctattcccagccaagttcctatacccagcaaagcggctatggacagca
agcctcatattctcagcagagttcttatgggcagccaagtagttacggccaacaaagtggatatggcccaacagaagtagctaccaagccacccccccca
aa
3' EST: CF785128 [+]tttttttttttttttcttttttttttttttaacccttaacaccaagttttattatacaaaaaacgtttacagaatagaagataaaagtatagagttaaac
ctgatgccattttttttggcacttaagtttacaatatgaatgtttacaaaacattgttctgttctaagttttatattattgactgatattgggatatttt
tcctctccatttagacaccgagtcccacttttcataatgctgccggctgagtgggcacttttaagaactgaagtgtatacaacgctctcactttgtaacc
ccatgccaccacgagagcccctcccaccacggctcatgccgcctcttccaaacatgccccgacccctgctgcgtgagtctgagcctccagaaccgcgatt
ctcgccagagctggagaaaccctgatggggctcctggccatagggacttttgctgctagagtgatctggacgatagctgctctgttgtccataactactg
ctctgctgcccatactgccctgggggctggctgctataggaagacaaaggtggtgggtagctggtgggaggaggttgttgctgttgtgggggtgcctggt
agctactctgttggccatatccactttgttggccgtaactacttggctgcccataagaactctgctgagaatatgaggcttgctgtccatagccgctttg
ctgggtataggaacttggctgggaataggagctctgctcgtagcttgatgtttgagagctcgagtancttgtangaaggtaggatggaggggcgctcact
tgctgcattggata
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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